Development and mapping of gene-tagged SNP markers in laccases of maize (Zea mays L.)

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • J R Andersen, Denmark
  • T Asp
  • Y C Lu, College of Agricultural Resources and Environmental Sciences, Heilongjiang University, China
  • M Kloiber-Maitz, KWS Saat AG, Germany
  • M Ouzonova, KWS Saat AG, Germany
  • T Luebberstedt, Department of Agronomy, Iowa State University, United States
  • Department of Genetics and Biotechnology
  • Molekylær Genetik og Bioteknologi
Laccases, EC 1.10.3.2 or p-diphenol : dioxygen oxidoreductases, have been proposed to be involved in the oxidative polymerization of monolignols into lignins in plants. While 17 laccases have been identified in Arabidopsis, only five (ZmLac1-5) have so far been identified in maize. By a bioinformatic approach, 14 putative laccases were identified in maize. One putative laccase was identical to ZmLac1, while five were highly homologous to either ZmLac4 or ZmLac5. Sequence alignment of allelic sequences enabled the development of TaqMan single nucleotide polymorphism (SNP) markers for nine putative laccases. Four of these gene-tagged SNP markers were validated in a doubled-haploid mapping population of 140 individuals, mapping these loci to chromosomes 1, 2, 3 and 7, respectively.
Original languageEnglish
JournalPlant Breeding
Volume128
Issue4
Pages (from-to)423-425
Number of pages3
ISSN0179-9541
DOIs
Publication statusPublished - 2009

    Research areas

  • Maize, Laccase, Lignin, SNP, Forage quality

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