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Development and assessment of GC/MS and HPAEC/PAD methodologies for the quantification of α-galacto-oligosaccharides (GOS) in dry beans (Phaseolus vulgaris)

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  • Joana Pico, University of Guelph
  • ,
  • Natalia P. Vidal, University of Guelph
  • ,
  • Listiya Widjaja, University of Guelph
  • ,
  • Louis Falardeau, Bonduelle Americas
  • ,
  • Lionel Albino, Bonduelle S.A.
  • ,
  • Mario M. Martinez

The quantification of α-Galacto-oligosaccharides (GOS) in beans has been increasingly approached through different methodologies. However, reported GOS contents revealed up to 8-times disparity, which cannot be only attributed to the bean cultivar and underlines the need of using validated analytical methodologies. This study aimed to optimize and validate the extraction of the most abundant GOS found in beans, namely raffinose, stachyose and verbascose, and comparatively assess their determination by High-Performance Anion Exchange Chromatography/Pulsed Amperometric Detector (HPAEC/PAD) and Gas Chromatography/Mass Spectrometry (GC/MS). Hot sonication followed by shaking with 70% ethanol resulted in excellent GOS extraction efficiencies (92.54–107.94%). GC/MS determination was more reliable than HPAEC/PAD, with limits of quantification of 4.48–224.31 mg/kg and intra/inter-day repeatabilities <10%. The analysis of six bean varieties proved the feasibility of the GC/MS methodology, displaying total GOS contents from 1453.07 ± 169.31 to 2814.34 ± 95.28 mg/100 g. Stachyose was significantly (p < 0.05) the main GOS in all samples.

Original languageEnglish
Article number129151
JournalFood Chemistry
Number of pages10
Publication statusPublished - Jul 2021

    Research areas

  • GC/MS, HPAEC/PAD, Raffinose, Stachyose, Trimethylsilyl derivatives, Verbascose

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