Detection of proteolytic signatures for Parkinson's disease

Peter Lüttge Jordal, Thomas F. Dyrlund, Kristian Winge, Martin R. Larsen, Erik H. Danielsen, James A. Wells, Daniel E. Otzen, Jan J. Enghild*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

Aim: To investigate if idiopathic Parkinson's disease (IPD) is associated with distinct proteolytic signatures relative to non-neurodegenerative controls (NND) and patients with multiple system atrophy (MSA). Materials & methods: A subtiligase-based N-terminomics screening method was exploited for semiquantitative comparison of protein N-termini in cerebrospinal fluid for pooled samples of IPD (n = 6) and NND (n = 8) individuals. Subsequently, targeted selected reaction monitoring mass spectrometry measured the relative concentration of the proteolytic signature peptides in individual IPD (n = 22), NND (n = 11) and MSA (n = 18) samples. Results: The discovery screen detected 300 N-termini for 156 proteins. Selected reaction monitoring analysis revealed that two of these peptides differentiate IPD from NND while three peptides differentiate IPD from MSA. Conclusion: IPD is associated with distinct proteolytic signatures.

Original languageEnglish
JournalFuture Neurology
Volume11
Issue1
Pages (from-to)15-32
Number of pages18
ISSN1479-6708
DOIs
Publication statusPublished - 1 Feb 2016

Keywords

  • cerebrospinal fluid
  • N-terminomics
  • Parkinson's disease

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