Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

DOI

  • Jun-Young Kim
  • ,
  • Srikanta Sahu, Denmark
  • Yin-Hoe Yau
  • ,
  • Xu Wang
  • ,
  • Susana Geifman Shochat
  • ,
  • Per Halkjær Nielsen
  • ,
  • Morten Simonsen Dueholm
  • ,
  • Daniel E Otzen
  • Jungyeol Lee, Denmark
  • May Margarette Salido Delos Santos, Denmark
  • Joey Kuok Hoong Yam, Denmark
  • Nam-Young Kang, Denmark
  • Sung-Jin Park, Denmark
  • Hawyoung Kwon, Denmark
  • Thomas William Seviour, Denmark
  • Liang Yang
  • ,
  • Michael Givskov
  • ,
  • Young-Tae Chang, Denmark

Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods which fail to detect non-culturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (Compound of Designation Yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a Diversity Oriented Fluorescent Library Approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.

Original languageEnglish
JournalJournal of the American Chemical Society
Pages (from-to)402-407
ISSN0002-7863
DOIs
Publication statusPublished - 2016

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