TY - JOUR
T1 - Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11
AU - Kim, Jun-Young
AU - Sahu, Srikanta
AU - Yau, Yin-Hoe
AU - Wang, Xu
AU - Shochat, Susana Geifman
AU - Nielsen, Per Halkjær
AU - Dueholm, Morten Simonsen
AU - Otzen, Daniel E
AU - Lee, Jungyeol
AU - Delos Santos, May Margarette Salido
AU - Yam, Joey Kuok Hoong
AU - Kang, Nam-Young
AU - Park, Sung-Jin
AU - Kwon, Hawyoung
AU - Seviour, Thomas William
AU - Yang, Liang
AU - Givskov, Michael
AU - Chang, Young-Tae
PY - 2016/1/13
Y1 - 2016/1/13
N2 - Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods which fail to detect non-culturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (Compound of Designation Yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a Diversity Oriented Fluorescent Library Approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.
AB - Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods which fail to detect non-culturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (Compound of Designation Yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a Diversity Oriented Fluorescent Library Approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.
UR - http://www.scopus.com/inward/record.url?scp=84954342145&partnerID=8YFLogxK
U2 - 10.1021/jacs.5b11357
DO - 10.1021/jacs.5b11357
M3 - Journal article
C2 - 26684612
SN - 0002-7863
VL - 138
SP - 402
EP - 407
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 1
ER -