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Depletion and enrichment of phytosterols in soybean oil lipid emulsions directly associate with serum markers of cholestasis in preterm parenteral nutrition–fed pigs

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DOI

  • Gregory Guthrie, Baylor College of Medicine
  • ,
  • Barbara Stoll, Baylor College of Medicine
  • ,
  • Shaji Chacko, Baylor College of Medicine, United States
  • Mahmoud Mohammas, Baylor College of Medicine
  • ,
  • Candace Style, Department of Pediatric Surgery, Nationwide Children’s Hospital, Columbus, Ohio
  • ,
  • Mariatu Verla, Department of Pediatric Surgery, Nationwide Children’s Hospital, Columbus, Ohio
  • ,
  • Oluyinka Olutoye, Department of Pediatric Surgery, Nationwide Children’s Hospital, Columbus, Ohio, United States
  • Deborah Schady, Department of Pathology, Baylor College of Medicine, Houston, Texas
  • ,
  • Charlotte Lauridsen
  • Nick Tataryn, Center for Comparative Medicine, Baylor College of Medicine, Houston, Texas, United States
  • Douglas G Burrin, Baylor College of Medicine, United States
Background
Clinical reports show a positive correlation between phytosterol concentrations and severity of cholestatic liver disease markers in infants during long-term administration of parenteral lipid emulsions. Establishing a causal link between phytosterols and cholestasis has been complicated by confounding factors of lipid emulsion load, fatty acid composition, and vitamin E in many of these studies. The goal of this study is to determine whether altering the phytosterol concentration within a common soybean oil–based emulsion will alter the onset and severity of cholestasis in parenterally fed preterm piglets.

Methods
Preterm piglets were administered, for 21 days, either enteral nutrition (ENT) or parenteral nutrition (PN) prepared from a soybean oil–based emulsion containing either 24.0% (depleted [DEP]), 100% (Intralipid; normal phytosterol [NP] concentration), or 144% (enriched [ENR]) total phytosterol concentration.

Results
At the end of the study, plasma and liver phytosterol concentrations were highest in the ENR group, followed by NP and then DEP and ENT. Serum direct bilirubin, serum bile acids, and γ-glutamyltransferase were higher in the ENR and NP groups compared with either DEP or ENT groups. All PN lipid groups showed evidence of mild hepatic steatosis but no change in hepatic expression of proinflammatory cytokines or Farnesoid X receptor target genes.

Conclusion
The increase in serum direct bilirubin was lower in the DEP group vs the lipid emulsions with normal or ENR phytosterols. Our results provide additional evidence that phytosterols are linked to an increase in serum markers of cholestasis in preterm PN-fed pigs.
Original languageEnglish
JournalJournal of Parenteral and Enteral Nutrition
Volume46
Issue1
Pages (from-to)160-171
Number of pages12
ISSN0148-6071
DOIs
Publication statusPublished - Jan 2022

    Research areas

  • bile acids, bile salt export pump, parenteral nutrition, parenteral nutrition-associated cholestasis, phytosterols, soybean oil, associated cholestasis, parenteral nutrition&#8211

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