Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings

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Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings. / Andersen, T.E.; Kolmos, H.J.; Palarasah, Yaseelan; Skjødt, Martin; Koch, C.; Benter, Maike; Alei, M.; Kingshott, P.; Ogaki, Ryosuke.

In: Biomaterials, Vol. 32, No. 20, 2011, p. 4481-4488.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Andersen, TE, Kolmos, HJ, Palarasah, Y, Skjødt, M, Koch, C, Benter, M, Alei, M, Kingshott, P & Ogaki, R 2011, 'Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings', Biomaterials, vol. 32, no. 20, pp. 4481-4488. https://doi.org/10.1016/j.biomaterials.2011.03.002

APA

Andersen, T. E., Kolmos, H. J., Palarasah, Y., Skjødt, M., Koch, C., Benter, M., ... Ogaki, R. (2011). Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings. Biomaterials, 32(20), 4481-4488. https://doi.org/10.1016/j.biomaterials.2011.03.002

CBE

Andersen TE, Kolmos HJ, Palarasah Y, Skjødt M, Koch C, Benter M, Alei M, Kingshott P, Ogaki R. 2011. Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings. Biomaterials. 32(20):4481-4488. https://doi.org/10.1016/j.biomaterials.2011.03.002

MLA

Vancouver

Author

Andersen, T.E. ; Kolmos, H.J. ; Palarasah, Yaseelan ; Skjødt, Martin ; Koch, C. ; Benter, Maike ; Alei, M. ; Kingshott, P. ; Ogaki, Ryosuke. / Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings. In: Biomaterials. 2011 ; Vol. 32, No. 20. pp. 4481-4488.

Bibtex

@article{d835dc04f5ad4da4b43758160d2f3fc4,
title = "Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings",
abstract = "In the current study we investigate the activation of blood complement on medical device silicone rubber and present a plasma polymerized vinyl pyrrolidone (ppVP) coating which strongly decreases surface-activation of the blood complement system. We show that uncoated silicone and polystyrene are both potent activators of the complement system, measured both as activated, deposited C3b and quantifying fluid-phase release of the cleavage fragment C3c. The ppVP coated silicone exhibits approximately 90{\%} reduced complement activation compared to untreated silicone. Quartz crystal microbalance with dissipation (QCM-D) measurements show relatively strong adsorption of blood proteins including native C3 to the ppVP surface, indicating that reduction of complement activation on ppVP is neither a result of low protein adsorption nor lower direct C3-binding, and is therefore possibly a consequence of differences in the adsorbed protein layer composition. The alternative and classical complement pathways are barely detectable on ppVP while the lectin pathway through MBL/ficolin-2 deposition remains active on ppVP suggesting this pathway is responsible for the remaining subtle activation on the ppVP coated surface. The ppVP surface is furthermore characterized physically and chemically using scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR), which indicates preservation of chemical functionality by the applied plasma process. Overall, the ppVP coating shows a potential for increasing complement-compatibility of blood-contacting devices.",
author = "T.E. Andersen and H.J. Kolmos and Yaseelan Palarasah and Martin Skj{\o}dt and C. Koch and Maike Benter and M. Alei and P. Kingshott and Ryosuke Ogaki",
note = "MEDLINE{\circledR} is the source for the MeSH terms of this document.",
year = "2011",
doi = "10.1016/j.biomaterials.2011.03.002",
language = "English",
volume = "32",
pages = "4481--4488",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "20",

}

RIS

TY - JOUR

T1 - Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings

AU - Andersen, T.E.

AU - Kolmos, H.J.

AU - Palarasah, Yaseelan

AU - Skjødt, Martin

AU - Koch, C.

AU - Benter, Maike

AU - Alei, M.

AU - Kingshott, P.

AU - Ogaki, Ryosuke

N1 - MEDLINE® is the source for the MeSH terms of this document.

PY - 2011

Y1 - 2011

N2 - In the current study we investigate the activation of blood complement on medical device silicone rubber and present a plasma polymerized vinyl pyrrolidone (ppVP) coating which strongly decreases surface-activation of the blood complement system. We show that uncoated silicone and polystyrene are both potent activators of the complement system, measured both as activated, deposited C3b and quantifying fluid-phase release of the cleavage fragment C3c. The ppVP coated silicone exhibits approximately 90% reduced complement activation compared to untreated silicone. Quartz crystal microbalance with dissipation (QCM-D) measurements show relatively strong adsorption of blood proteins including native C3 to the ppVP surface, indicating that reduction of complement activation on ppVP is neither a result of low protein adsorption nor lower direct C3-binding, and is therefore possibly a consequence of differences in the adsorbed protein layer composition. The alternative and classical complement pathways are barely detectable on ppVP while the lectin pathway through MBL/ficolin-2 deposition remains active on ppVP suggesting this pathway is responsible for the remaining subtle activation on the ppVP coated surface. The ppVP surface is furthermore characterized physically and chemically using scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR), which indicates preservation of chemical functionality by the applied plasma process. Overall, the ppVP coating shows a potential for increasing complement-compatibility of blood-contacting devices.

AB - In the current study we investigate the activation of blood complement on medical device silicone rubber and present a plasma polymerized vinyl pyrrolidone (ppVP) coating which strongly decreases surface-activation of the blood complement system. We show that uncoated silicone and polystyrene are both potent activators of the complement system, measured both as activated, deposited C3b and quantifying fluid-phase release of the cleavage fragment C3c. The ppVP coated silicone exhibits approximately 90% reduced complement activation compared to untreated silicone. Quartz crystal microbalance with dissipation (QCM-D) measurements show relatively strong adsorption of blood proteins including native C3 to the ppVP surface, indicating that reduction of complement activation on ppVP is neither a result of low protein adsorption nor lower direct C3-binding, and is therefore possibly a consequence of differences in the adsorbed protein layer composition. The alternative and classical complement pathways are barely detectable on ppVP while the lectin pathway through MBL/ficolin-2 deposition remains active on ppVP suggesting this pathway is responsible for the remaining subtle activation on the ppVP coated surface. The ppVP surface is furthermore characterized physically and chemically using scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR), which indicates preservation of chemical functionality by the applied plasma process. Overall, the ppVP coating shows a potential for increasing complement-compatibility of blood-contacting devices.

UR - http://www.scopus.com/inward/record.url?scp=79955523569&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2011.03.002

DO - 10.1016/j.biomaterials.2011.03.002

M3 - Journal article

C2 - 21453967

AN - SCOPUS:79955523569

VL - 32

SP - 4481

EP - 4488

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 20

ER -