Decoding Atomic Addresses: Solution NMR Resonance Assignment of Proteins

Thibault Viennet, Abhinav Dubey, Ricarda Törner, Maxim Droemer, Paul Coote, Dominique Frueh, Koh Takeuchi, Haribabu Arthanari

Research output: Contribution to book/anthology/report/proceedingBook chapterResearch

Abstract

NMR is a powerful analytical technique that permits the exploration of biomolecules under physiological conditions with atomic resolution. It is especially applicable for examining protein structures and their interactions and dynamics in environments closely resembling their native state, extending its utility to uniquely study disordered proteins. Nevertheless, to extract atomic resolution details, one must successfully correlate observed resonances with their originating nuclei, a process known as ‘resonance assignment’. Even with over fifty years of technical advancements, resonance assignment frequently becomes a bottleneck in the utilization of NMR for the comprehensive study of structure, dynamics, and interactions. In this context, we delve into both the traditional methods and the emerging frontiers in protein resonance assignment strategies for solution NMR. Our goal is to provide a comprehensive view of the existing experimental methodologies, with a focused discussion on their strengths and potential limitations. In this chapter, we will strictly focus on resonance assignment strategies for proteins.
Original languageEnglish
Title of host publicationIntegrated Structural Biology
EditorsTatyana Polenova, Caitlin Quinn, Angela Gronenborn
Number of pages42
Place of publicationLondon
PublisherThe Royal Society of Chemistry
Publication dateDec 2023
Pages1-42
Chapter1
ISBN (Print)978-1-83916-183-4
ISBN (Electronic)978-1-83767-016-1, 978-1-83767-015-4
DOIs
Publication statusPublished - Dec 2023
SeriesNew Developments in NMR
Volume30
ISSN2044-253X

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