Dechlorination and organohalide-respiring bacteria dynamics in sediment samples of the Yangtze Three Gorges Reservoir

Irene Kranzioch; Claudia Stoll; Andreas Holbach; Hao Chen; Lijing Wang; Binghui Zheng; Stefan Norra; Yonghong Bi; Karl Werner Schramm; Andreas Tiehm

doi:10.1007/s11356-013-1545-9

Dechlorination and organohalide-respiring bacteria dynamics in sediment samples of the Yangtze Three Gorges Reservoir

Irene Kranzioch, Claudia Stoll, Andreas Holbach, Hao Chen, Lijing Wang, Binghui Zheng, Stefan Norra, Yonghong Bi, Karl Werner Schramm, Andreas Tiehm^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

45 Citations (Scopus)

Abstract

Several groups of bacteria such as Dehalococcoides spp., Dehalobacter spp., Desulfomonile spp., Desulfuromonas spp., or Desulfitobacterium spp. are able to dehalogenate chlorinated pollutants such as chloroethenes, chlorobenzenes, or polychlorinated biphenyls under anaerobic conditions. In order to assess the dechlorination potential in Yangtze sediment samples, the presence and activity of the reductively dechlorinating bacteria were studied in anaerobic batch tests. Eighteen sediment samples were taken in the Three Gorges Reservoir catchment area of the Yangtze River, including the tributaries Jialing River, Daning River, and Xiangxi River. Polymerase chain reaction analysis indicated the presence of dechlorinating bacteria in most samples, with varying dechlorinating microbial community compositions at different sampling locations. Subsequently, anaerobic reductive dechlorination of tetrachloroethene (PCE) was tested after the addition of electron donors. Most cultures dechlorinated PCE completely to ethene via cis-dichloroethene (cis-DCE) or trans-dichloroethene. Dehalogenating activity corresponded to increasing numbers of Dehalobacter spp., Desulfomonile spp., Desulfitobacterium spp., or Dehalococcoides spp. If no bacteria of the genus Dehalococcoides spp. were present in the sediment, reductive dechlorination stopped at cis-DCE. Our results demonstrate the presence of viable dechlorinating bacteria in Yangtze samples, indicating their relevance for pollutant turnover.

Original language	English
Journal	Environmental Science and Pollution Research
Volume	20
Issue	10
Pages (from-to)	7046-7056
Number of pages	11
ISSN	0944-1344
DOIs	https://doi.org/10.1007/s11356-013-1545-9
Publication status	Published - Oct 2013
Externally published	Yes

Keywords

Dehalococcoides spp.
Desulfitobacterium spp.
qPCR
Reductive dechlorination
Tetrachloroethene
Yangtze

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10.1007/s11356-013-1545-9

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@article{532eaed736304454a2855d41c9190b94,

title = "Dechlorination and organohalide-respiring bacteria dynamics in sediment samples of the Yangtze Three Gorges Reservoir",

abstract = "Several groups of bacteria such as Dehalococcoides spp., Dehalobacter spp., Desulfomonile spp., Desulfuromonas spp., or Desulfitobacterium spp. are able to dehalogenate chlorinated pollutants such as chloroethenes, chlorobenzenes, or polychlorinated biphenyls under anaerobic conditions. In order to assess the dechlorination potential in Yangtze sediment samples, the presence and activity of the reductively dechlorinating bacteria were studied in anaerobic batch tests. Eighteen sediment samples were taken in the Three Gorges Reservoir catchment area of the Yangtze River, including the tributaries Jialing River, Daning River, and Xiangxi River. Polymerase chain reaction analysis indicated the presence of dechlorinating bacteria in most samples, with varying dechlorinating microbial community compositions at different sampling locations. Subsequently, anaerobic reductive dechlorination of tetrachloroethene (PCE) was tested after the addition of electron donors. Most cultures dechlorinated PCE completely to ethene via cis-dichloroethene (cis-DCE) or trans-dichloroethene. Dehalogenating activity corresponded to increasing numbers of Dehalobacter spp., Desulfomonile spp., Desulfitobacterium spp., or Dehalococcoides spp. If no bacteria of the genus Dehalococcoides spp. were present in the sediment, reductive dechlorination stopped at cis-DCE. Our results demonstrate the presence of viable dechlorinating bacteria in Yangtze samples, indicating their relevance for pollutant turnover.",

keywords = "Dehalococcoides spp., Desulfitobacterium spp., qPCR, Reductive dechlorination, Tetrachloroethene, Yangtze",

author = "Irene Kranzioch and Claudia Stoll and Andreas Holbach and Hao Chen and Lijing Wang and Binghui Zheng and Stefan Norra and Yonghong Bi and Schramm, {Karl Werner} and Andreas Tiehm",

note = "Funding Information: Acknowledgments The authors gratefully acknowledge the financial support from the German Ministry of Education and Research (BMBF, grant no.: 02WT1130). This study is part of the Sino-German Yangtze-Hydro Project (www.yangtze-project.de).",

year = "2013",

month = oct,

doi = "10.1007/s11356-013-1545-9",

language = "English",

volume = "20",

pages = "7046--7056",

journal = "Environmental Science and Pollution Research",

issn = "0944-1344",

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Dechlorination and organohalide-respiring bacteria dynamics in sediment samples of the Yangtze Three Gorges Reservoir. / Kranzioch, Irene; Stoll, Claudia; Holbach, Andreas et al.
In: Environmental Science and Pollution Research, Vol. 20, No. 10, 10.2013, p. 7046-7056.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Dechlorination and organohalide-respiring bacteria dynamics in sediment samples of the Yangtze Three Gorges Reservoir

AU - Kranzioch, Irene

AU - Stoll, Claudia

AU - Holbach, Andreas

AU - Chen, Hao

AU - Wang, Lijing

AU - Zheng, Binghui

AU - Norra, Stefan

AU - Bi, Yonghong

AU - Schramm, Karl Werner

AU - Tiehm, Andreas

N1 - Funding Information: Acknowledgments The authors gratefully acknowledge the financial support from the German Ministry of Education and Research (BMBF, grant no.: 02WT1130). This study is part of the Sino-German Yangtze-Hydro Project (www.yangtze-project.de).

PY - 2013/10

Y1 - 2013/10

N2 - Several groups of bacteria such as Dehalococcoides spp., Dehalobacter spp., Desulfomonile spp., Desulfuromonas spp., or Desulfitobacterium spp. are able to dehalogenate chlorinated pollutants such as chloroethenes, chlorobenzenes, or polychlorinated biphenyls under anaerobic conditions. In order to assess the dechlorination potential in Yangtze sediment samples, the presence and activity of the reductively dechlorinating bacteria were studied in anaerobic batch tests. Eighteen sediment samples were taken in the Three Gorges Reservoir catchment area of the Yangtze River, including the tributaries Jialing River, Daning River, and Xiangxi River. Polymerase chain reaction analysis indicated the presence of dechlorinating bacteria in most samples, with varying dechlorinating microbial community compositions at different sampling locations. Subsequently, anaerobic reductive dechlorination of tetrachloroethene (PCE) was tested after the addition of electron donors. Most cultures dechlorinated PCE completely to ethene via cis-dichloroethene (cis-DCE) or trans-dichloroethene. Dehalogenating activity corresponded to increasing numbers of Dehalobacter spp., Desulfomonile spp., Desulfitobacterium spp., or Dehalococcoides spp. If no bacteria of the genus Dehalococcoides spp. were present in the sediment, reductive dechlorination stopped at cis-DCE. Our results demonstrate the presence of viable dechlorinating bacteria in Yangtze samples, indicating their relevance for pollutant turnover.

AB - Several groups of bacteria such as Dehalococcoides spp., Dehalobacter spp., Desulfomonile spp., Desulfuromonas spp., or Desulfitobacterium spp. are able to dehalogenate chlorinated pollutants such as chloroethenes, chlorobenzenes, or polychlorinated biphenyls under anaerobic conditions. In order to assess the dechlorination potential in Yangtze sediment samples, the presence and activity of the reductively dechlorinating bacteria were studied in anaerobic batch tests. Eighteen sediment samples were taken in the Three Gorges Reservoir catchment area of the Yangtze River, including the tributaries Jialing River, Daning River, and Xiangxi River. Polymerase chain reaction analysis indicated the presence of dechlorinating bacteria in most samples, with varying dechlorinating microbial community compositions at different sampling locations. Subsequently, anaerobic reductive dechlorination of tetrachloroethene (PCE) was tested after the addition of electron donors. Most cultures dechlorinated PCE completely to ethene via cis-dichloroethene (cis-DCE) or trans-dichloroethene. Dehalogenating activity corresponded to increasing numbers of Dehalobacter spp., Desulfomonile spp., Desulfitobacterium spp., or Dehalococcoides spp. If no bacteria of the genus Dehalococcoides spp. were present in the sediment, reductive dechlorination stopped at cis-DCE. Our results demonstrate the presence of viable dechlorinating bacteria in Yangtze samples, indicating their relevance for pollutant turnover.

KW - Dehalococcoides spp.

KW - Desulfitobacterium spp.

KW - qPCR

KW - Reductive dechlorination

KW - Tetrachloroethene

KW - Yangtze

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DO - 10.1007/s11356-013-1545-9

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SN - 0944-1344

VL - 20

SP - 7046

EP - 7056

JO - Environmental Science and Pollution Research

JF - Environmental Science and Pollution Research

IS - 10

ER -

Dechlorination and organohalide-respiring bacteria dynamics in sediment samples of the Yangtze Three Gorges Reservoir

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Keywords

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