Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli

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  • Lisa Gerner, University of Oslo
  • ,
  • Steffi Munack, University of Oslo
  • ,
  • Koen Temmerman, European Molecular Biology Laboratory Heidelberg
  • ,
  • Ann Marie Lawrence-Dörner, Westfälische Wilhelms-Universität Münster
  • ,
  • Hüseyin Besir, European Molecular Biology Laboratory Heidelberg
  • ,
  • Matthias Wilmanns, European Molecular Biology Laboratory Hamburg
  • ,
  • Jan Kristian Jensen
  • Bernd Thiede, Universitetet i Oslo
  • ,
  • Ian G. Mills, Queen's University, Belfast
  • ,
  • Jens Preben Morth, Centre for Molecular Medicine Norway (NCMM), University of Oslo, Institute for Experimental Medical Research

Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 'apo', CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required and the proteins subsequently used to develop a fluorescence-based assay for drug binding to the kinase, "Using the fluorescent properties of STO-609 as a tool to assist structure-function analyses of recombinant CaMKK2" [1].

Original languageEnglish
JournalData in Brief
Volume8
Pages (from-to)733-740
Number of pages8
ISSN2352-3409
DOIs
Publication statusPublished - 1 Sep 2016

    Research areas

  • Calmodulin, CaMKK2, Fermentation

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