Cultured mast cells from asthmatic patients and controls respond with similar sensitivity to recombinant Der P2 induced, IgE-mediated activation

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  • Inge Jacoba Maria Kortekaas Krohn, Denmark
  • Asger Sverrild, Department of Respiratory Medicine L, Bispebjerg University Hospital, Denmark
  • Gitte Lund, ALK-Abello, Hørsholm, Denmark
  • Ronald Dahl
  • Jonas S Erjefält, University of Lund, Sweden
  • Vibeke Backer, Department of Respiratory Medicine L, Bispebjerg University Hospital, Denmark
  • Hans Jürgen Hoffmann
The function of cultured mast cells may depend on genetic or environmental influence on the stem cell donor. This study investigates whether asthma or atopy in the donor influenced the growth and sensitivity of mast cells cultured from asthma patients and healthy controls under identical conditions. Mast cells were cultured from peripheral blood from twelve patients with an objectively confirmed asthma diagnosis and eight healthy subjects. During the last 2 weeks of culture, mast cells were incubated with IL-4 and 80 kU/L recombinant human IgE containing two clones (7%+7%) specific for mite allergen Der p2. The sensitivity of IgE-mediated activation of mast cells was investigated as FcεRI-mediated up-regulation of CD63. Ten subjects were atopic, defined as a positive skin prick test (>3 mm) to at least one of ten common allergens. After activation with recombinant Der p2, the maximum CD63 Median Fluorescence Intensity was 20 456 ± 1640 (SE) for asthma patients and 22 275 ± 1971 (SE) for controls (ns). The fraction of CD63 positive cells was 54.4% in asthma patients and 48.4% in controls (ns). The allergen concentration inducing 50% of the maximal CD63 response was similar in asthma patients (-0.4795 log ng/mL ± 0.092 (SE)) and controls (-0.6351 log ng/mL ± 0.083, ns) and in atopic and non-atopic subjects. When cultured, sensitised and activated under identical conditions, mast cells from allergic asthmatics and healthy controls respond similar. Activation of cultured mast cells appears to depend on culture conditions (IL-4, IgE) rather than on donor status as atopy and asthma. This article is protected by copyright. All rights reserved.
Original languageEnglish
JournalScandinavian Journal of Immunology
Volume78
Issue4
Pages (from-to)352-6
ISSN0300-9475
DOIs
Publication statusPublished - 24 Jun 2013

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