Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection

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  • nbt.4110

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  • Hungate1000 Project Collaborators

Productivity of ruminant livestock depends on the rumen microbiota, which ferment indigestible plant polysaccharides into nutrients used for growth. Understanding the functions carried out by the rumen microbiota is important for reducing greenhouse gas production by ruminants and for developing biofuels from lignocellulose. We present 410 cultured bacteria and archaea, together with their reference genomes, representing every cultivated rumen-associated archaeal and bacterial family. We evaluate polysaccharide degradation, short-chain fatty acid production and methanogenesis pathways, and assign specific taxa to functions. A total of 336 organisms were present in available rumen metagenomic data sets, and 134 were present in human gut microbiome data sets. Comparison with the human microbiome revealed rumen-specific enrichment for genes encoding de novo synthesis of vitamin B-12, ongoing evolution by gene loss and potential vertical inheritance of the rumen microbiome based on underrepresentation of markers of environmental stress. We estimate that our Hungate genome resource represents similar to 75% of the genus-level bacterial and archaeal taxa present in the rumen.

Original languageEnglish
JournalNature Biotechnology
Volume36
Issue4
Pages (from-to)359-367
Number of pages11
ISSN1087-0156
DOIs
Publication statusPublished - Apr 2018

    Research areas

  • RUMINANT NUTRITION, REDUCING EMISSIONS, REFERENCE GENOMES, DAIRY-CATTLE, BACTERIAL, DATABASE, REVEALS, ANNOTATION, DIVERSITY, EVOLUTION

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