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Crystallisation and preliminary X-ray analysis of the receptor-binding domain of human and bovine alpha 2-macroglobulin

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  • Department of Molecular Biology
  • Department of Molecular Biology
The receptor-binding domains (RBDs) of human and bovine alpha 2-macroglobulin (alpha 2M) have been isolated after limited proteolysis of methylamine-treated alpha 2M with papain. Single crystals of the RBDs have been grown by vapour diffusion. Crystals of human RBD are very thin plates unsuited for data collection. However, crystals of RBD from bovine alpha 2M give diffraction patterns suitable for X-ray analysis, and a complete dataset with a maximum resolution of 2.3 A has been collected with synchrotron radiation at cryogenic temperature. The crystals belong to spacegroup P3(1)21 or P3(2)21 with cell parameters a = b = 106.8 A, c = 72.2 A.
Original languageEnglish
JournalF E B S Letters
Pages (from-to)93-5
Number of pages2
Publication statusPublished - 1995

    Research areas

  • Amino Acid Sequence, Animals, Binding Sites, Cattle, Crystallization, Crystallography, X-Ray, Humans, LDL-Receptor Related Protein 1, Molecular Sequence Data, Peptide Fragments, Receptors, Immunologic, Receptors, LDL, Sequence Alignment, alpha-Macroglobulins

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