Crystal structure of a eukaryotic phosphate transporter

Bjørn Panyella Pedersen, Hemant Kumar, Andrew B Waight, Aaron J Risenmay, Zygy Roe-Zurz, Bryant H Chau, Avner Schlessinger, Massimiliano Bonomi, William Harries, Andrej Sali, Atul K Johri, Robert M Stroud

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

    Abstract

    Phosphate is crucial for structural and metabolic needs, including nucleotide and lipid synthesis, signalling and chemical energy storage. Proton-coupled transporters of the major facilitator superfamily (MFS) are essential for phosphate uptake in plants and fungi, and also have a function in sensing external phosphate levels as transceptors. Here we report the 2.9 Å structure of a fungal (Piriformospora indica) high-affinity phosphate transporter, PiPT, in an inward-facing occluded state, with bound phosphate visible in the membrane-buried binding site. The structure indicates both proton and phosphate exit pathways and suggests a modified asymmetrical 'rocker-switch' mechanism of phosphate transport. PiPT is related to several human transporter families, most notably the organic cation and anion transporters of the solute carrier family (SLC22), which are implicated in cancer-drug resistance. We modelled representative cation and anion SLC22 transporters based on the PiPT structure to surmise the structural basis for substrate binding and charge selectivity in this important family. The PiPT structure demonstrates and expands on principles of substrate transport by the MFS transporters and illuminates principles of phosphate uptake in particular.

    Original languageEnglish
    JournalNature
    Volume496
    Issue7446
    Pages (from-to)533-536
    Number of pages4
    ISSN0028-0836
    DOIs
    Publication statusPublished - 25 Apr 2013

    Keywords

    • Basidiomycota
    • Binding Sites
    • Crystallography, X-Ray
    • Eukaryotic Cells
    • Humans
    • Models, Biological
    • Models, Molecular
    • Phosphate Transport Proteins
    • Phosphates
    • Protein Conformation
    • Protons
    • Structure-Activity Relationship

    Fingerprint

    Dive into the research topics of 'Crystal structure of a eukaryotic phosphate transporter'. Together they form a unique fingerprint.

    Cite this