Controlling Styrene Maleic Acid Lipid Particles through RAFT

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Anton A. A. Smith
  • Henriette E. Autzen, Department of Biochemistry and Biophysics, University of California, San Francisco
  • ,
  • Tomas Laursen, Univ Copenhagen, University of Copenhagen, Dept Plant & Environm Sci PLEN, Lawrence Berkeley National Laboratory, CA
  • ,
  • Vincent Wu, Univ Calif Berkeley, University of California Berkeley, University of California System, Chem Mat Sci & Engn
  • ,
  • Max Yen, Univ Calif Berkeley, University of California Berkeley, University of California System, Chem Mat Sci & Engn
  • ,
  • Aaron Hall, Univ Calif Berkeley, University of California Berkeley, University of California System, Chem Mat Sci & Engn
  • ,
  • Scott D. Hansen, Univ Calif Berkeley, University of California Berkeley, University of California System, Calif Inst Quantitat Biosci QB3
  • ,
  • Yifan Cheng, UCSF, University of California San Francisco, University of California System, Biochem & Biophys
  • ,
  • Ting Xu, Univ Calif Berkeley, University of California Berkeley, University of California System, Chem Mat Sci & Engn

The ability of styrene maleic acid copolymers to dissolve lipid membranes into nanosized lipid particles is a facile method of obtaining membrane proteins in solubilized lipid discs while conserving part of their native lipid environment. While the currently used copolymers can readily extract membrane proteins in native nanodiscs, their highly disperse composition is likely to influence the dispersity of the discs as well as the extraction efficiency. In this study, reversible addition-fragmentation chain transfer was used to control the polymer architecture and dispersity of molecular weights with a high-precision. Based on Monte Carlo simulations of the polymerizations, the monomer composition was predicted and allowed a structure function analysis of the polymer architecture, in relation to their ability to assemble into lipid nanoparticles. We show that a higher degree of control of the polymer architecture generates more homogeneous samples. We hypothesize that low dispersity copolymers, with control of polymer architecture are an ideal framework for the rational design of polymers for customized isolation and characterization of integral membrane proteins in native lipid bilayer systems.

Original languageEnglish
JournalBiomacromolecules
Volume18
Issue11
Pages (from-to)3706-3713
Number of pages8
ISSN1525-7797
DOIs
Publication statusPublished - Nov 2017
Event4th Symposium on Innovative Polymers for Controlled Delivery (SIPCD) - Suzhou
Duration: 23 Sep 201626 Sep 2016

Conference

Conference4th Symposium on Innovative Polymers for Controlled Delivery (SIPCD)
CitySuzhou
Period23/09/201626/09/2016

    Research areas

  • FRAGMENTATION CHAIN TRANSFER, MEMBRANE-PROTEINS, NANODISCS, COPOLYMER, ANHYDRIDE, POLYMERIZATION, SOLUBILIZATION, NANOPARTICLES, MONOMERS, BEHAVIOR

See relations at Aarhus University Citationformats

ID: 121328699