Control of enzyme reactions by a reconfigurable DNA nanovault

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Control of enzyme reactions by a reconfigurable DNA nanovault. / Grossi, Guido; Jepsen, Mette Dalgaard Ebbesen; Kjems, Jorgen; Andersen, Ebbe Sloth.

In: Nature Communications, Vol. 8, 992, 19.10.2017.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{4bd8c9e6613e4a23a23ec9ef55049709,
title = "Control of enzyme reactions by a reconfigurable DNA nanovault",
abstract = "Biological systems use compartmentalisation as a general strategy to control enzymatic reactions by precisely regulating enzyme-substrate interactions. With the advent of DNA nanotechnology, it has become possible to rationally design DNA-based nano-containers with programmable structural and dynamic properties. These DNA nanostructures have been used to cage enzymes, but control over enzyme-substrate interactions using a dynamic DNA nanostructure has not been achieved yet. Here we introduce a DNA origami device that functions as a nanoscale vault: an enzyme is loaded in an isolated cavity and the access to free substrate molecules is controlled by a multi-lock mechanism. The DNA vault is characterised for features such as reversible opening/closing, cargo loading and wall porosity, and is shown to control the enzymatic reaction catalysed by an encapsulated protease. The DNA vault represents a general concept to control enzyme-substrate interactions by inducing conformational changes in a rationally designed DNA nanodevice.",
keywords = "STRAND-DISPLACEMENT, ORIGAMI, PROTEIN, ENCAPSULATION, CONSTRUCTION, NANOREACTOR, SHAPES, COMPARTMENTALIZATION, NANOSTRUCTURES, TETRAHEDRON",
author = "Guido Grossi and Jepsen, {Mette Dalgaard Ebbesen} and Jorgen Kjems and Andersen, {Ebbe Sloth}",
year = "2017",
month = oct,
day = "19",
doi = "10.1038/s41467-017-01072-8",
language = "English",
volume = "8",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Control of enzyme reactions by a reconfigurable DNA nanovault

AU - Grossi, Guido

AU - Jepsen, Mette Dalgaard Ebbesen

AU - Kjems, Jorgen

AU - Andersen, Ebbe Sloth

PY - 2017/10/19

Y1 - 2017/10/19

N2 - Biological systems use compartmentalisation as a general strategy to control enzymatic reactions by precisely regulating enzyme-substrate interactions. With the advent of DNA nanotechnology, it has become possible to rationally design DNA-based nano-containers with programmable structural and dynamic properties. These DNA nanostructures have been used to cage enzymes, but control over enzyme-substrate interactions using a dynamic DNA nanostructure has not been achieved yet. Here we introduce a DNA origami device that functions as a nanoscale vault: an enzyme is loaded in an isolated cavity and the access to free substrate molecules is controlled by a multi-lock mechanism. The DNA vault is characterised for features such as reversible opening/closing, cargo loading and wall porosity, and is shown to control the enzymatic reaction catalysed by an encapsulated protease. The DNA vault represents a general concept to control enzyme-substrate interactions by inducing conformational changes in a rationally designed DNA nanodevice.

AB - Biological systems use compartmentalisation as a general strategy to control enzymatic reactions by precisely regulating enzyme-substrate interactions. With the advent of DNA nanotechnology, it has become possible to rationally design DNA-based nano-containers with programmable structural and dynamic properties. These DNA nanostructures have been used to cage enzymes, but control over enzyme-substrate interactions using a dynamic DNA nanostructure has not been achieved yet. Here we introduce a DNA origami device that functions as a nanoscale vault: an enzyme is loaded in an isolated cavity and the access to free substrate molecules is controlled by a multi-lock mechanism. The DNA vault is characterised for features such as reversible opening/closing, cargo loading and wall porosity, and is shown to control the enzymatic reaction catalysed by an encapsulated protease. The DNA vault represents a general concept to control enzyme-substrate interactions by inducing conformational changes in a rationally designed DNA nanodevice.

KW - STRAND-DISPLACEMENT

KW - ORIGAMI

KW - PROTEIN

KW - ENCAPSULATION

KW - CONSTRUCTION

KW - NANOREACTOR

KW - SHAPES

KW - COMPARTMENTALIZATION

KW - NANOSTRUCTURES

KW - TETRAHEDRON

U2 - 10.1038/s41467-017-01072-8

DO - 10.1038/s41467-017-01072-8

M3 - Journal article

C2 - 29051565

VL - 8

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 992

ER -