Control of enzyme reactions by a reconfigurable DNA nanovault

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Documents

DOI

Biological systems use compartmentalisation as a general strategy to control enzymatic reactions by precisely regulating enzyme-substrate interactions. With the advent of DNA nanotechnology, it has become possible to rationally design DNA-based nano-containers with programmable structural and dynamic properties. These DNA nanostructures have been used to cage enzymes, but control over enzyme-substrate interactions using a dynamic DNA nanostructure has not been achieved yet. Here we introduce a DNA origami device that functions as a nanoscale vault: an enzyme is loaded in an isolated cavity and the access to free substrate molecules is controlled by a multi-lock mechanism. The DNA vault is characterised for features such as reversible opening/closing, cargo loading and wall porosity, and is shown to control the enzymatic reaction catalysed by an encapsulated protease. The DNA vault represents a general concept to control enzyme-substrate interactions by inducing conformational changes in a rationally designed DNA nanodevice.

Original languageEnglish
Article number992
JournalNature Communications
Volume8
Number of pages8
ISSN2041-1723
DOIs
Publication statusPublished - 19 Oct 2017

    Research areas

  • STRAND-DISPLACEMENT, ORIGAMI, PROTEIN, ENCAPSULATION, CONSTRUCTION, NANOREACTOR, SHAPES, COMPARTMENTALIZATION, NANOSTRUCTURES, TETRAHEDRON

See relations at Aarhus University Citationformats

Download statistics

No data available

ID: 118602513