Control of enzyme reactions by a reconfigurable DNA nanovault

Guido Grossi, Mette Dalgaard Ebbesen Jepsen, Jorgen Kjems, Ebbe Sloth Andersen*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

147 Citations (Scopus)
274 Downloads (Pure)

Abstract

Biological systems use compartmentalisation as a general strategy to control enzymatic reactions by precisely regulating enzyme-substrate interactions. With the advent of DNA nanotechnology, it has become possible to rationally design DNA-based nano-containers with programmable structural and dynamic properties. These DNA nanostructures have been used to cage enzymes, but control over enzyme-substrate interactions using a dynamic DNA nanostructure has not been achieved yet. Here we introduce a DNA origami device that functions as a nanoscale vault: an enzyme is loaded in an isolated cavity and the access to free substrate molecules is controlled by a multi-lock mechanism. The DNA vault is characterised for features such as reversible opening/closing, cargo loading and wall porosity, and is shown to control the enzymatic reaction catalysed by an encapsulated protease. The DNA vault represents a general concept to control enzyme-substrate interactions by inducing conformational changes in a rationally designed DNA nanodevice.

Original languageEnglish
Article number992
JournalNature Communications
Volume8
Issue1
Number of pages8
ISSN2041-1723
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • STRAND-DISPLACEMENT
  • ORIGAMI
  • PROTEIN
  • ENCAPSULATION
  • CONSTRUCTION
  • NANOREACTOR
  • SHAPES
  • COMPARTMENTALIZATION
  • NANOSTRUCTURES
  • TETRAHEDRON

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