Contractile benefits of doublet-initiated low-frequency stimulation in rat extensor digitorum longus muscle exposed to high extracellular [K+] or fatiguing contractions

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Contractile benefits of doublet-initiated low-frequency stimulation in rat extensor digitorum longus muscle exposed to high extracellular [K+] or fatiguing contractions. / Pedersen, Katja Krustrup; Nielsen, Ole Bækgaard; Overgaard, Kristian.

In: American journal of physiology. Cell physiology, Vol. 317, No. 1, 2019, p. C39-C47.

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@article{f3289286139f4475bd79d5ffaa6bddda,
title = "Contractile benefits of doublet-initiated low-frequency stimulation in rat extensor digitorum longus muscle exposed to high extracellular [K+] or fatiguing contractions",
abstract = "During dynamic contractions, high-frequency muscle activation is needed to achieve optimal power. This must be balanced against an increased excitation-induced accumulation of extracellular K+, which can reduce excitability and ultimately may prevent adequate responses to high-frequency activation. Mean activation frequencies in vivo are often low (subtetanic), but activation patterns contain bursts of high (supratetanic) frequencies known as doublets, which enhance dynamic contraction in rested muscles at normal extracellular K+ concentration ([K+]o). Here, we examine how dynamic contractions in fast-twitch fibers stimulated by high frequency/doublets are affected during exposure to 11 mM [K+]o and during fatigue. Dynamic contractions were elicited by electrical stimulation in isolated rat extensor digitorum longus muscles incubated at 4 or 11 mM K+. When stimulation frequency was maintained constant, an increase from 150 to 300 Hz enhanced maximal power (Pmax), maximal velocity (Vmax), and rate of force development (RFD) at 4 mM K+ but only Vmax at 11 mM K+. With the use of subtetanic frequency trains (50 Hz) with or without an initiating doublet (300 Hz), the addition of a doublet increased maximal force, Pmax, Vmax, and RFD at both 4 and 11 mM K+. Furthermore, a work-matched fatiguing protocol was performed comparing a doublet-initiated subtetanic train (DT) of 60 Hz with a constant-frequency train (CFT) of 71 Hz during 100 dynamic contractions. We found that DT produced higher power, velocity, and RFD than CFT throughout the fatiguing protocol. The results indicate that doublets enhance dynamic contraction in fast-twitch muscles stimulated at subtetanic frequency during both normal and fatiguing conditions.",
keywords = "doublets, elevated extracellular potassium, fatigue, power, rate of force development",
author = "Pedersen, {Katja Krustrup} and Nielsen, {Ole B{\ae}kgaard} and Kristian Overgaard",
year = "2019",
doi = "10.1152/ajpcell.00519.2018",
language = "English",
volume = "317",
pages = "C39--C47",
journal = "American Journal of Physiology: Cell Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "1",

}

RIS

TY - JOUR

T1 - Contractile benefits of doublet-initiated low-frequency stimulation in rat extensor digitorum longus muscle exposed to high extracellular [K+] or fatiguing contractions

AU - Pedersen, Katja Krustrup

AU - Nielsen, Ole Bækgaard

AU - Overgaard, Kristian

PY - 2019

Y1 - 2019

N2 - During dynamic contractions, high-frequency muscle activation is needed to achieve optimal power. This must be balanced against an increased excitation-induced accumulation of extracellular K+, which can reduce excitability and ultimately may prevent adequate responses to high-frequency activation. Mean activation frequencies in vivo are often low (subtetanic), but activation patterns contain bursts of high (supratetanic) frequencies known as doublets, which enhance dynamic contraction in rested muscles at normal extracellular K+ concentration ([K+]o). Here, we examine how dynamic contractions in fast-twitch fibers stimulated by high frequency/doublets are affected during exposure to 11 mM [K+]o and during fatigue. Dynamic contractions were elicited by electrical stimulation in isolated rat extensor digitorum longus muscles incubated at 4 or 11 mM K+. When stimulation frequency was maintained constant, an increase from 150 to 300 Hz enhanced maximal power (Pmax), maximal velocity (Vmax), and rate of force development (RFD) at 4 mM K+ but only Vmax at 11 mM K+. With the use of subtetanic frequency trains (50 Hz) with or without an initiating doublet (300 Hz), the addition of a doublet increased maximal force, Pmax, Vmax, and RFD at both 4 and 11 mM K+. Furthermore, a work-matched fatiguing protocol was performed comparing a doublet-initiated subtetanic train (DT) of 60 Hz with a constant-frequency train (CFT) of 71 Hz during 100 dynamic contractions. We found that DT produced higher power, velocity, and RFD than CFT throughout the fatiguing protocol. The results indicate that doublets enhance dynamic contraction in fast-twitch muscles stimulated at subtetanic frequency during both normal and fatiguing conditions.

AB - During dynamic contractions, high-frequency muscle activation is needed to achieve optimal power. This must be balanced against an increased excitation-induced accumulation of extracellular K+, which can reduce excitability and ultimately may prevent adequate responses to high-frequency activation. Mean activation frequencies in vivo are often low (subtetanic), but activation patterns contain bursts of high (supratetanic) frequencies known as doublets, which enhance dynamic contraction in rested muscles at normal extracellular K+ concentration ([K+]o). Here, we examine how dynamic contractions in fast-twitch fibers stimulated by high frequency/doublets are affected during exposure to 11 mM [K+]o and during fatigue. Dynamic contractions were elicited by electrical stimulation in isolated rat extensor digitorum longus muscles incubated at 4 or 11 mM K+. When stimulation frequency was maintained constant, an increase from 150 to 300 Hz enhanced maximal power (Pmax), maximal velocity (Vmax), and rate of force development (RFD) at 4 mM K+ but only Vmax at 11 mM K+. With the use of subtetanic frequency trains (50 Hz) with or without an initiating doublet (300 Hz), the addition of a doublet increased maximal force, Pmax, Vmax, and RFD at both 4 and 11 mM K+. Furthermore, a work-matched fatiguing protocol was performed comparing a doublet-initiated subtetanic train (DT) of 60 Hz with a constant-frequency train (CFT) of 71 Hz during 100 dynamic contractions. We found that DT produced higher power, velocity, and RFD than CFT throughout the fatiguing protocol. The results indicate that doublets enhance dynamic contraction in fast-twitch muscles stimulated at subtetanic frequency during both normal and fatiguing conditions.

KW - doublets

KW - elevated extracellular potassium

KW - fatigue

KW - power

KW - rate of force development

UR - http://www.scopus.com/inward/record.url?scp=85067085043&partnerID=8YFLogxK

U2 - 10.1152/ajpcell.00519.2018

DO - 10.1152/ajpcell.00519.2018

M3 - Journal article

C2 - 30969780

AN - SCOPUS:85067085043

VL - 317

SP - C39-C47

JO - American Journal of Physiology: Cell Physiology

JF - American Journal of Physiology: Cell Physiology

SN - 0363-6143

IS - 1

ER -