Confocal microscopy imaging of the biofilm matrix

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Confocal microscopy imaging of the biofilm matrix. / Schlafer, Sebastian; Meyer, Rikke L.

In: Journal of Microbiological Methods, Vol. 138, 07.2017, p. 50-59.

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Schlafer, Sebastian ; Meyer, Rikke L. / Confocal microscopy imaging of the biofilm matrix. In: Journal of Microbiological Methods. 2017 ; Vol. 138. pp. 50-59.

Bibtex

@article{cfce6ca2c52b467da962804af78d6dfe,
title = "Confocal microscopy imaging of the biofilm matrix",
abstract = "The extracellular matrix is an integral part of microbial biofilms and an important field of research. Confocal laser scanning microscopy is a valuable tool for the study of biofilms, and in particular of the biofilm matrix, as it allows real-time visualization of fully hydrated, living specimens. Confocal microscopes are held by many research groups, and a number of methods for qualitative and quantitative imaging of the matrix have emerged in recent years. This review provides an overview and a critical discussion of techniques used to visualize different matrix compounds, to determine the concentration of solutes and the diffusive properties of the biofilm matrix.",
author = "Sebastian Schlafer and Meyer, {Rikke L}",
note = "Copyright {\circledC} 2016 Elsevier B.V. All rights reserved.",
year = "2017",
month = "7",
doi = "10.1016/j.mimet.2016.03.002",
language = "English",
volume = "138",
pages = "50--59",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Confocal microscopy imaging of the biofilm matrix

AU - Schlafer, Sebastian

AU - Meyer, Rikke L

N1 - Copyright © 2016 Elsevier B.V. All rights reserved.

PY - 2017/7

Y1 - 2017/7

N2 - The extracellular matrix is an integral part of microbial biofilms and an important field of research. Confocal laser scanning microscopy is a valuable tool for the study of biofilms, and in particular of the biofilm matrix, as it allows real-time visualization of fully hydrated, living specimens. Confocal microscopes are held by many research groups, and a number of methods for qualitative and quantitative imaging of the matrix have emerged in recent years. This review provides an overview and a critical discussion of techniques used to visualize different matrix compounds, to determine the concentration of solutes and the diffusive properties of the biofilm matrix.

AB - The extracellular matrix is an integral part of microbial biofilms and an important field of research. Confocal laser scanning microscopy is a valuable tool for the study of biofilms, and in particular of the biofilm matrix, as it allows real-time visualization of fully hydrated, living specimens. Confocal microscopes are held by many research groups, and a number of methods for qualitative and quantitative imaging of the matrix have emerged in recent years. This review provides an overview and a critical discussion of techniques used to visualize different matrix compounds, to determine the concentration of solutes and the diffusive properties of the biofilm matrix.

U2 - 10.1016/j.mimet.2016.03.002

DO - 10.1016/j.mimet.2016.03.002

M3 - Review

VL - 138

SP - 50

EP - 59

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

ER -