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Conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis

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Conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis. / Kelly, Simon; Muszyński, Artur; Kawaharada, Yasuyuki; Hubber, Andree M; Sullivan, John T; Sandal, Niels; Carlson, Russell W; Stougaard, Jens; Ronson, Clive W.

In: Molecular Plant - Microbe Interactions, Vol. 26, No. 3, 03.2013, p. 319-329.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Kelly, S, Muszyński, A, Kawaharada, Y, Hubber, AM, Sullivan, JT, Sandal, N, Carlson, RW, Stougaard, J & Ronson, CW 2013, 'Conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis', Molecular Plant - Microbe Interactions, vol. 26, no. 3, pp. 319-329. https://doi.org/10.1094/MPMI-09-12-0227-R

APA

Kelly, S., Muszyński, A., Kawaharada, Y., Hubber, A. M., Sullivan, J. T., Sandal, N., Carlson, R. W., Stougaard, J., & Ronson, C. W. (2013). Conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis. Molecular Plant - Microbe Interactions, 26(3), 319-329. https://doi.org/10.1094/MPMI-09-12-0227-R

CBE

Kelly S, Muszyński A, Kawaharada Y, Hubber AM, Sullivan JT, Sandal N, Carlson RW, Stougaard J, Ronson CW. 2013. Conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis. Molecular Plant - Microbe Interactions. 26(3):319-329. https://doi.org/10.1094/MPMI-09-12-0227-R

MLA

Vancouver

Kelly S, Muszyński A, Kawaharada Y, Hubber AM, Sullivan JT, Sandal N et al. Conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis. Molecular Plant - Microbe Interactions. 2013 Mar;26(3):319-329. https://doi.org/10.1094/MPMI-09-12-0227-R

Author

Kelly, Simon ; Muszyński, Artur ; Kawaharada, Yasuyuki ; Hubber, Andree M ; Sullivan, John T ; Sandal, Niels ; Carlson, Russell W ; Stougaard, Jens ; Ronson, Clive W. / Conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis. In: Molecular Plant - Microbe Interactions. 2013 ; Vol. 26, No. 3. pp. 319-329.

Bibtex

@article{65404fde7ec746f9a8bd77f479dcc1f3,
title = "Conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis",
abstract = "Rhizobial surface polysaccharides are required for nodule formation on the roots of at least some legumes but the mechanisms by which they act are yet to be determined. As a first step to investigate the function of exopolysaccharide (EPS) in the formation of determinate nodules, we isolated Mesorhizobium loti mutants affected in various steps of EPS biosynthesis and characterized their symbiotic phenotypes on two Lotus spp. The wild-type M. loti R7A produced both high molecular weight EPS and lower molecular weight (LMW) polysaccharide fractions whereas most mutant strains produced only LMW fractions. Mutants affected in predicted early biosynthetic steps (e.g., exoB) formed nitrogen-fixing nodules on Lotus corniculatus and L. japonicus 'Gifu', whereas mutants affected in mid or late biosynthetic steps (e.g., exoU) induced uninfected nodule primordia and, occasionally, a few infected nodules following a lengthy delay. These mutants were disrupted at the stage of infection thread (IT) development. Symbiotically defective EPS and Nod factor mutants functionally complemented each other in co-inoculation experiments. The majority of full-length IT observed harbored only the EPS mutant strain and did not show bacterial release, whereas the nitrogen-fixing nodules contained both mutants. Examination of the symbiotic proficiency of the exoU mutant on various L. japonicus ecotypes revealed that both host and environmental factors were linked to the requirement for EPS. These results reveal a complex function for M. loti EPS in determinate nodule formation and suggest that EPS plays a signaling role at the stages of both IT initiation and bacterial release.",
author = "Simon Kelly and Artur Muszy{\'n}ski and Yasuyuki Kawaharada and Hubber, {Andree M} and Sullivan, {John T} and Niels Sandal and Carlson, {Russell W} and Jens Stougaard and Ronson, {Clive W}",
year = "2013",
month = mar,
doi = "10.1094/MPMI-09-12-0227-R",
language = "English",
volume = "26",
pages = "319--329",
journal = "Molecular Plant - Microbe Interactions",
issn = "0894-0282",
publisher = "AMER PHYTOPATHOLOGICAL SOC",
number = "3",

}

RIS

TY - JOUR

T1 - Conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis

AU - Kelly, Simon

AU - Muszyński, Artur

AU - Kawaharada, Yasuyuki

AU - Hubber, Andree M

AU - Sullivan, John T

AU - Sandal, Niels

AU - Carlson, Russell W

AU - Stougaard, Jens

AU - Ronson, Clive W

PY - 2013/3

Y1 - 2013/3

N2 - Rhizobial surface polysaccharides are required for nodule formation on the roots of at least some legumes but the mechanisms by which they act are yet to be determined. As a first step to investigate the function of exopolysaccharide (EPS) in the formation of determinate nodules, we isolated Mesorhizobium loti mutants affected in various steps of EPS biosynthesis and characterized their symbiotic phenotypes on two Lotus spp. The wild-type M. loti R7A produced both high molecular weight EPS and lower molecular weight (LMW) polysaccharide fractions whereas most mutant strains produced only LMW fractions. Mutants affected in predicted early biosynthetic steps (e.g., exoB) formed nitrogen-fixing nodules on Lotus corniculatus and L. japonicus 'Gifu', whereas mutants affected in mid or late biosynthetic steps (e.g., exoU) induced uninfected nodule primordia and, occasionally, a few infected nodules following a lengthy delay. These mutants were disrupted at the stage of infection thread (IT) development. Symbiotically defective EPS and Nod factor mutants functionally complemented each other in co-inoculation experiments. The majority of full-length IT observed harbored only the EPS mutant strain and did not show bacterial release, whereas the nitrogen-fixing nodules contained both mutants. Examination of the symbiotic proficiency of the exoU mutant on various L. japonicus ecotypes revealed that both host and environmental factors were linked to the requirement for EPS. These results reveal a complex function for M. loti EPS in determinate nodule formation and suggest that EPS plays a signaling role at the stages of both IT initiation and bacterial release.

AB - Rhizobial surface polysaccharides are required for nodule formation on the roots of at least some legumes but the mechanisms by which they act are yet to be determined. As a first step to investigate the function of exopolysaccharide (EPS) in the formation of determinate nodules, we isolated Mesorhizobium loti mutants affected in various steps of EPS biosynthesis and characterized their symbiotic phenotypes on two Lotus spp. The wild-type M. loti R7A produced both high molecular weight EPS and lower molecular weight (LMW) polysaccharide fractions whereas most mutant strains produced only LMW fractions. Mutants affected in predicted early biosynthetic steps (e.g., exoB) formed nitrogen-fixing nodules on Lotus corniculatus and L. japonicus 'Gifu', whereas mutants affected in mid or late biosynthetic steps (e.g., exoU) induced uninfected nodule primordia and, occasionally, a few infected nodules following a lengthy delay. These mutants were disrupted at the stage of infection thread (IT) development. Symbiotically defective EPS and Nod factor mutants functionally complemented each other in co-inoculation experiments. The majority of full-length IT observed harbored only the EPS mutant strain and did not show bacterial release, whereas the nitrogen-fixing nodules contained both mutants. Examination of the symbiotic proficiency of the exoU mutant on various L. japonicus ecotypes revealed that both host and environmental factors were linked to the requirement for EPS. These results reveal a complex function for M. loti EPS in determinate nodule formation and suggest that EPS plays a signaling role at the stages of both IT initiation and bacterial release.

U2 - 10.1094/MPMI-09-12-0227-R

DO - 10.1094/MPMI-09-12-0227-R

M3 - Journal article

C2 - 23134480

VL - 26

SP - 319

EP - 329

JO - Molecular Plant - Microbe Interactions

JF - Molecular Plant - Microbe Interactions

SN - 0894-0282

IS - 3

ER -