Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes

Caroline Chylinski; Kristin Fløgstad Degnes; Inga Marie Aasen; Sokratis Ptochos; Berit Marie Blomstrand; Karl Christian Mahnert; Heidi Larsen Enemark; Stig Milan Thamsborg; Håvard Steinshamn; Spiridoula Athanasiadou

doi:10.1038/s41598-023-38476-0

Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes

Caroline Chylinski, Kristin Fløgstad Degnes, Inga Marie Aasen, Sokratis Ptochos, Berit Marie Blomstrand, Karl Christian Mahnert, Heidi Larsen Enemark, Stig Milan Thamsborg, Håvard Steinshamn, Spiridoula Athanasiadou^*

^*Corresponding author for this work

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Abstract

The antiparasitic potential of plants could offer a vital solution to alleviating the costs of gastrointestinal nematode (GIN) infections in ruminant production globally. Leveraging known bioactive molecules, however, is complex, where plant species, extraction processes and seasonality impact bioavailability and efficacy. This study assessed the impact of a comprehensive set of factors on the antiparasitic activity of Norwegian conifers to identify bark compounds specific against GIN. Antiparasitic activity was determined using in vitro assays targeting morphologically distinct life stages of ovine GIN: the egg hatch assay and larval motility assay. In depth characterisation of the chemical composition of the bark extracts was carried out using chromatographic separation, UV-absorbance, and molecular mass profiles to identify compounds implicated in the activity. Three key findings emerged: (1) the activity of bark extracts varied markedly from 0 to 100% antiparasitic efficacy, owing to tree species, extraction solvent and seasonality; (2) the GIN exhibited species-and stage-specific susceptibility to the bark extracts; (3) the presence of condensed tannins, amongst other compounds, was associated with anthelmintic activity. These findings add new insights into urgently needed alternative parasite control strategies in livestock.

Original language	English
Article number	13498
Journal	Scientific Reports
Volume	13
Issue	1
Number of pages	12
ISSN	2045-2322
DOIs	https://doi.org/10.1038/s41598-023-38476-0
Publication status	Published - Dec 2023
Externally published	Yes

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2023_Chylinski et al._CT novel compounds sources of variationFinal published version, 1.52 MB

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Chylinski, C., Degnes, K. F., Aasen, I. M., Ptochos, S., Blomstrand, B. M., Mahnert, K. C., Enemark, H. L., Thamsborg, S. M., Steinshamn, H., & Athanasiadou, S. (2023). Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes. Scientific Reports, 13(1), Article 13498. https://doi.org/10.1038/s41598-023-38476-0

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title = "Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes",

abstract = "The antiparasitic potential of plants could offer a vital solution to alleviating the costs of gastrointestinal nematode (GIN) infections in ruminant production globally. Leveraging known bioactive molecules, however, is complex, where plant species, extraction processes and seasonality impact bioavailability and efficacy. This study assessed the impact of a comprehensive set of factors on the antiparasitic activity of Norwegian conifers to identify bark compounds specific against GIN. Antiparasitic activity was determined using in vitro assays targeting morphologically distinct life stages of ovine GIN: the egg hatch assay and larval motility assay. In depth characterisation of the chemical composition of the bark extracts was carried out using chromatographic separation, UV-absorbance, and molecular mass profiles to identify compounds implicated in the activity. Three key findings emerged: (1) the activity of bark extracts varied markedly from 0 to 100% antiparasitic efficacy, owing to tree species, extraction solvent and seasonality; (2) the GIN exhibited species-and stage-specific susceptibility to the bark extracts; (3) the presence of condensed tannins, amongst other compounds, was associated with anthelmintic activity. These findings add new insights into urgently needed alternative parasite control strategies in livestock.",

author = "Caroline Chylinski and Degnes, {Kristin Fl{\o}gstad} and Aasen, {Inga Marie} and Sokratis Ptochos and Blomstrand, {Berit Marie} and Mahnert, {Karl Christian} and Enemark, {Heidi Larsen} and Thamsborg, {Stig Milan} and H{\aa}vard Steinshamn and Spiridoula Athanasiadou",

year = "2023",

month = dec,

doi = "10.1038/s41598-023-38476-0",

language = "English",

volume = "13",

journal = "Scientific Reports",

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Chylinski, C, Degnes, KF, Aasen, IM, Ptochos, S, Blomstrand, BM, Mahnert, KC, Enemark, HL, Thamsborg, SM, Steinshamn, H & Athanasiadou, S 2023, 'Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes', Scientific Reports, vol. 13, no. 1, 13498. https://doi.org/10.1038/s41598-023-38476-0

Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes. / Chylinski, Caroline; Degnes, Kristin Fløgstad; Aasen, Inga Marie et al.
In: Scientific Reports, Vol. 13, No. 1, 13498, 12.2023.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes

AU - Chylinski, Caroline

AU - Degnes, Kristin Fløgstad

AU - Aasen, Inga Marie

AU - Ptochos, Sokratis

AU - Blomstrand, Berit Marie

AU - Mahnert, Karl Christian

AU - Enemark, Heidi Larsen

AU - Thamsborg, Stig Milan

AU - Steinshamn, Håvard

AU - Athanasiadou, Spiridoula

PY - 2023/12

Y1 - 2023/12

N2 - The antiparasitic potential of plants could offer a vital solution to alleviating the costs of gastrointestinal nematode (GIN) infections in ruminant production globally. Leveraging known bioactive molecules, however, is complex, where plant species, extraction processes and seasonality impact bioavailability and efficacy. This study assessed the impact of a comprehensive set of factors on the antiparasitic activity of Norwegian conifers to identify bark compounds specific against GIN. Antiparasitic activity was determined using in vitro assays targeting morphologically distinct life stages of ovine GIN: the egg hatch assay and larval motility assay. In depth characterisation of the chemical composition of the bark extracts was carried out using chromatographic separation, UV-absorbance, and molecular mass profiles to identify compounds implicated in the activity. Three key findings emerged: (1) the activity of bark extracts varied markedly from 0 to 100% antiparasitic efficacy, owing to tree species, extraction solvent and seasonality; (2) the GIN exhibited species-and stage-specific susceptibility to the bark extracts; (3) the presence of condensed tannins, amongst other compounds, was associated with anthelmintic activity. These findings add new insights into urgently needed alternative parasite control strategies in livestock.

AB - The antiparasitic potential of plants could offer a vital solution to alleviating the costs of gastrointestinal nematode (GIN) infections in ruminant production globally. Leveraging known bioactive molecules, however, is complex, where plant species, extraction processes and seasonality impact bioavailability and efficacy. This study assessed the impact of a comprehensive set of factors on the antiparasitic activity of Norwegian conifers to identify bark compounds specific against GIN. Antiparasitic activity was determined using in vitro assays targeting morphologically distinct life stages of ovine GIN: the egg hatch assay and larval motility assay. In depth characterisation of the chemical composition of the bark extracts was carried out using chromatographic separation, UV-absorbance, and molecular mass profiles to identify compounds implicated in the activity. Three key findings emerged: (1) the activity of bark extracts varied markedly from 0 to 100% antiparasitic efficacy, owing to tree species, extraction solvent and seasonality; (2) the GIN exhibited species-and stage-specific susceptibility to the bark extracts; (3) the presence of condensed tannins, amongst other compounds, was associated with anthelmintic activity. These findings add new insights into urgently needed alternative parasite control strategies in livestock.

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DO - 10.1038/s41598-023-38476-0

M3 - Journal article

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AN - SCOPUS:85168359374

SN - 2045-2322

VL - 13

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 13498

ER -

Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes

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