Computer-Aided Design and Production of RNA Origami as Protein Scaffolds and Biosensors

Néstor Sampedro Vallina, Cody Geary, Mette Jepsen, Ebbe Sloth Andersen

Research output: Contribution to book/anthology/report/proceedingBook chapterResearch

Abstract

RNA nanotechnology is able to take advantage of the modularity of RNA to build a wide variety of structures and functional devices from a common set of structural modules. The RNA origami architecture harnesses the property of RNA to fold as it is being enzymatically synthesized by the RNA polymerase and enables the design of single-stranded devices that integrate multiple structural and functional RNA motifs. Here, we provide detailed procedures on how to design and characterize RNA origami structures. The process is illustrated by two examples: one that forms lattices and another example that acts as biosensors.

Original languageEnglish
Title of host publicationDNA and RNA Origami : Methods and protocols
EditorsJulián Valero
Number of pages17
PublisherHumana Press
Publication date2023
Pages51-67
ISBN (Print)978-1-0716-3027-3
ISBN (Electronic)978-1-0716-3028-0
DOIs
Publication statusPublished - 2023
SeriesMethods in Molecular Biology
Volume2639
ISSN1064-3745

Keywords

  • Biosensing Techniques
  • Computer-Aided Design
  • DNA/chemistry
  • Nanostructures/chemistry
  • Nanotechnology/methods
  • Nucleic Acid Conformation
  • Proteins
  • RNA/chemistry

Fingerprint

Dive into the research topics of 'Computer-Aided Design and Production of RNA Origami as Protein Scaffolds and Biosensors'. Together they form a unique fingerprint.

Cite this