Comprehensive quantification of flavonoids and salicylic acid representative of Salix spp. using microLiquid Chromatography-Triple Quadrupole Mass Spectrometry: the importance of drying procedures and extraction solvent when performing classical solid-liquid extraction

TY - JOUR

T1 - Comprehensive quantification of flavonoids and salicylic acid representative of Salix spp. using microLiquid Chromatography-Triple Quadrupole Mass Spectrometry

T2 - the importance of drying procedures and extraction solvent when performing classical solid-liquid extraction

AU - Curtasu, Mihai Victor

AU - Nørskov, Natalja P.

N1 - Publisher Copyright: © 2023 The Authors

PY - 2023/8

Y1 - 2023/8

N2 - Willow (Salix spp.) is gaining an increasing interest as a fast-growing tree with high biomass yield from low agricultural inputs, which contains potentially bioactive compounds. The present work aimed to develop a high-yield extraction procedure combined with robust, sensitive and fast microLiquid Chromatography-Triple Quadrupole Mass Spectrometry (LC-MS/MS) based method for comprehensively quantifying flavonoids and salicylic acid in the bark of Salix spp. We have investigated the effect of freeze- and oven-drying procedures and five extraction solvents on the yield of individual flavonoid and salicylic acid when performing classical solid-liquid extraction. The freeze-drying was the best drying procedure for preserving monomeric and polymeric flavan-3-ols, whereas other flavonoids were less affected. Salicylic acid was not affected by the drying procedures. The best extraction solvent in terms of the yield of individual flavonoid among the tested solvents in this study was the combination of methanol acidified with 1% hydrochloric acid. LC-MS/MS method has shown a high recovery percentage (≥80%), good precision and overall robustness.

AB - Willow (Salix spp.) is gaining an increasing interest as a fast-growing tree with high biomass yield from low agricultural inputs, which contains potentially bioactive compounds. The present work aimed to develop a high-yield extraction procedure combined with robust, sensitive and fast microLiquid Chromatography-Triple Quadrupole Mass Spectrometry (LC-MS/MS) based method for comprehensively quantifying flavonoids and salicylic acid in the bark of Salix spp. We have investigated the effect of freeze- and oven-drying procedures and five extraction solvents on the yield of individual flavonoid and salicylic acid when performing classical solid-liquid extraction. The freeze-drying was the best drying procedure for preserving monomeric and polymeric flavan-3-ols, whereas other flavonoids were less affected. Salicylic acid was not affected by the drying procedures. The best extraction solvent in terms of the yield of individual flavonoid among the tested solvents in this study was the combination of methanol acidified with 1% hydrochloric acid. LC-MS/MS method has shown a high recovery percentage (≥80%), good precision and overall robustness.

KW - Flavonoids

KW - LC-MS/MS

KW - Phenolics

KW - Solid-liquid extraction

KW - Willow

UR - http://www.scopus.com/inward/record.url?scp=85161965871&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2023.464139

DO - 10.1016/j.chroma.2023.464139

M3 - Journal article

C2 - 37352692

AN - SCOPUS:85161965871

SN - 0021-9673

VL - 1705

JO - Journal of Chromatography A

JF - Journal of Chromatography A

M1 - 464139

ER -