Comprehensive genome methylation analysis in bladder cancer;identification and validation of novel methylated genes and application of these as urinary tumor markers

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PURPOSE: Epigenetic alterations are common and can now be addressed in a parallel fashion. We investigated the methylation in bladder cancer with respect to location in genome, consistency, variation in metachronous tumors, impact on transcripts, chromosomal location, and usefulness as urinary markers.EXPERIMENTAL DESIGN: A microarray assay was utilized to analyze methylation in 56 samples. Independent validation was performed in 63 samples by a PCR based method and bisulfite sequencing. The methylation levels in 174 urine specimens were quantified. Transcript levels were analyzed using expression microarrays, and pathways using dedicated software. RESULTS: Global methylation patterns were established within and outside CpG-islands. We validated methylation of the eight tumor markers genes ZNF154 (p
Original languageEnglish
JournalClinical Cancer Research
Publication statusPublished - 25 Jul 2011

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