Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies

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  • Ivana Domljanovic, Univ. of Southern Denmark
  • ,
  • Annika Carstens, Univ. of Southern Denmark
  • ,
  • Anders Okholm
  • ,
  • Jorgen Kjems
  • Christoffer Tandrup Nielsen, Copenhagen University Hospital, Copenhagen
  • ,
  • Niels H. H. Heegaard, STATENS SERUM INST, Univ. of Southern Denmark
  • ,
  • Kira Astakhova, Univ. of Southern Denmark

To date, there are multiple assays developed that detect and quantify antibodies in biofluids. Nevertheless, there is still a lack of simple approaches that specifically detect autoimmune antibodies to double-stranded DNA. Herein we investigate the potential of novel nucleic acid complexes as targets for these antibodies. This is done in a simple, rapid and specific immunofluorescence assay. Specifically, employing 3D nanostructures (DNA origami), we present a new approach in the detection and study of human antibodies to DNA. We demonstrate the detection of anti-DNA antibodies that are characteristic of systemic lupus erythematosus, a chronic autoimmune disease with multiple manifestations. We tested the most potent non-covalent pairs of DNA and fluorescent dyes. Several complexes showed specific recognition of autoimmune antibodies in human samples of lupus patients using a simple one-step immunofluorescence method. This makes the novel assay developed herein a promising tool for research and point-of-care monitoring of anti-DNA antibodies. Using this method, we for the first time experimentally confirm that the disease-specific autoimmune antibodies are sensitive to the 3D structure of nucleic acids and not only to the nucleotide sequence, as was previously thought.

Original languageEnglish
Article number1925
JournalScientific Reports
Volume7
Number of pages9
ISSN2045-2322
DOIs
Publication statusPublished - 15 May 2017

    Research areas

  • SYSTEMIC-LUPUS-ERYTHEMATOSUS, RHEUMATOID-ARTHRITIS, ANTINUCLEAR ANTIBODY, CLINICAL-PRACTICE, REVISED CRITERIA, CLASSIFICATION, DISEASE, AUTOANTIBODIES, DIAGNOSIS, PATTERNS

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