Competitive amplification of differentially melting amplicons (CADMA) improves KRAS hotspot mutation testing in colorectal cancer

TY - JOUR

T1 - Competitive amplification of differentially melting amplicons (CADMA) improves KRAS hotspot mutation testing in colorectal cancer

AU - Kristensen, Lasse Sommer

AU - Kjeldsen, Tina Ellegaard

AU - Hager, Henrik

AU - Hansen, Lise Lotte

PY - 2012

Y1 - 2012

N2 - Cancer is an extremely heterogeneous group of diseases traditionally categorized according to tissue of origin. However, even among patients with the same cancer subtype the cellular alterations at the molecular level are often very different. Several new therapies targeting specific molecular changes found in individual patients have initiated the era of personalized therapy and significantly improved patient care. In metastatic colorectal cancer (mCRC) a selected group of patients with wild-type KRAS respond to antibodies against the epidermal growth factor receptor (EGFR). Testing for KRAS mutations is now required prior to anti-EGFR treatment, however, less sensitive methods based on conventional PCR regularly fail to detect KRAS mutations in clinical samples.

AB - Cancer is an extremely heterogeneous group of diseases traditionally categorized according to tissue of origin. However, even among patients with the same cancer subtype the cellular alterations at the molecular level are often very different. Several new therapies targeting specific molecular changes found in individual patients have initiated the era of personalized therapy and significantly improved patient care. In metastatic colorectal cancer (mCRC) a selected group of patients with wild-type KRAS respond to antibodies against the epidermal growth factor receptor (EGFR). Testing for KRAS mutations is now required prior to anti-EGFR treatment, however, less sensitive methods based on conventional PCR regularly fail to detect KRAS mutations in clinical samples.

KW - Cell Line, Tumor

KW - Colorectal Neoplasms

KW - DNA Mutational Analysis

KW - DNA, Neoplasm

KW - Humans

KW - Mutation

KW - Polymerase Chain Reaction

KW - Proto-Oncogene Proteins

KW - Reproducibility of Results

KW - Sequence Analysis, DNA

KW - ras Proteins

U2 - 10.1186/1471-2407-12-548

DO - 10.1186/1471-2407-12-548

M3 - Journal article

C2 - 23173730

SN - 1471-2407

VL - 12

SP - 548

JO - B M C Cancer

JF - B M C Cancer

ER -