Comparing SRM and SWATH Methods for Quantitation of Bovine Muscle Proteomes

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DOI

  • Wei Wu, College of Food Science and Nutritional Engineering, China Agricultural University
  • ,
  • Rui Tong Dai, China Agricultural University
  • ,
  • Emøke Bendixen

Mass spectrometry (MS) has become essential for efficient and accurate quantification of proteins and proteomes and, thus, a key technology throughout all biosciences. However, validated MS methods are still scarce for meat quality research applications. The objective of this work was to develop and compare two targeted proteomic methods, namely, selected reaction monitoring (SRM) and sequential window acquisition of all theoretical spectra (SWATH), for the quantification of 11 bovine muscle proteins that may be indicators of meat color. Both methods require evaluation of spectra from proteotypic and quantotypic peptides, and we here report our evaluation of which peptides and MS parameters are best suited for robust quantification of these 11 proteins. We observed that the SRM approach provides better reproducibility, linearity, and sensitivity than SWATH and is therefore ideal for targeted quantification of low-abundance proteins, while the SWATH approach provides a more time-efficient method for targeted protein quantification of high-abundance proteins and, additionally, supports the search for novel biomarkers.

Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Volume67
Issue5
Pages (from-to)1608-1618
Number of pages11
ISSN0021-8561
DOIs
Publication statusPublished - 9 Jan 2019

    Research areas

  • Bos taurus, label-free quantitation, meat color, proteomics, SRM, SWATH

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