Comparing effects of nutrient sources approved for organic seaweed production on hatchery stage development of sugar kelp, Saccharina latissima

TY - JOUR

T1 - Comparing effects of nutrient sources approved for organic seaweed production on hatchery stage development of sugar kelp, Saccharina latissima

AU - Boderskov, Teis

AU - Rasmussen, Michael Bo

AU - Cassard, Christian Hein

AU - Svensgaard, Julie

AU - Enevoldsen, Laurids Nørskov

AU - Bruhn, Annette

PY - 2022/1

Y1 - 2022/1

N2 - The demand for organic certified seaweed is growing in European countries, but only few studies have investigated how nutrient sources approved for organic seaweed farming affect the growth and development of macroalgae. Through a series of four experiments, this study documented the effects of nitrogen (N) source and concentration on the key hatchery processes of meiospore germination, gametogenesis and initial sporophyte growth of Saccharina latissima. The nutrient sources were Provasoli Enriched Seawater (PES) with NO 3-N or NH 4-N and six potential organic certified growth media with N predominantly as NH 4-N, all with N concentrations up to 150 μM N. The potential organic certified N sources were: Degassed manure, protamylasse, three commercial liquid fertilizers and mussel excreted ammonium. In contrast to gametogenesis and initial sporophyte growth, the germination of S. latissima meiospores was generally not affected by neither N source nor concentration. Gametogenesis was the process being most sensitive to N source and concentration: In PES media, the N concentration had an overall positive effect on gametogenesis, albeit at concentrations of 150 μM NH 4, gametogenesis was impaired. The degassed manure, one liquid fertilizer and mussel excreted ammonium supported successful gametogenesis and sporophyte growth, but resulted in a 4.7, 20 and 1.3 fold lower sporophyte density after 23 days of growth, respectively, as compared to cultures growing with NO 3-PES. Optimum N concentrations were 50–100 μM N. We conclude that degassed manure and mussel excreted ammonium in concentrations <100 μM NH 4-N, are useful, but suboptimal N sources, for an organic hatchery production of S. latissima.

AB - The demand for organic certified seaweed is growing in European countries, but only few studies have investigated how nutrient sources approved for organic seaweed farming affect the growth and development of macroalgae. Through a series of four experiments, this study documented the effects of nitrogen (N) source and concentration on the key hatchery processes of meiospore germination, gametogenesis and initial sporophyte growth of Saccharina latissima. The nutrient sources were Provasoli Enriched Seawater (PES) with NO 3-N or NH 4-N and six potential organic certified growth media with N predominantly as NH 4-N, all with N concentrations up to 150 μM N. The potential organic certified N sources were: Degassed manure, protamylasse, three commercial liquid fertilizers and mussel excreted ammonium. In contrast to gametogenesis and initial sporophyte growth, the germination of S. latissima meiospores was generally not affected by neither N source nor concentration. Gametogenesis was the process being most sensitive to N source and concentration: In PES media, the N concentration had an overall positive effect on gametogenesis, albeit at concentrations of 150 μM NH 4, gametogenesis was impaired. The degassed manure, one liquid fertilizer and mussel excreted ammonium supported successful gametogenesis and sporophyte growth, but resulted in a 4.7, 20 and 1.3 fold lower sporophyte density after 23 days of growth, respectively, as compared to cultures growing with NO 3-PES. Optimum N concentrations were 50–100 μM N. We conclude that degassed manure and mussel excreted ammonium in concentrations <100 μM NH 4-N, are useful, but suboptimal N sources, for an organic hatchery production of S. latissima.

KW - Ammonium

KW - Degassed manure

KW - Gametogenesis

KW - Mussel ammonia excretion

KW - Organic fertilizers

KW - Sporophyte development

U2 - 10.1016/j.algal.2021.102602

DO - 10.1016/j.algal.2021.102602

M3 - Journal article

SN - 2211-9264

VL - 61

JO - Algal Research

JF - Algal Research

M1 - 102602

ER -