Projects per year
Abstract
Conventional identification based on morphology is today's tool of choice for monitoring earthworm diversity. However, it requires skills to distinguish specific features, which can become the bottleneck in soil ecological studies. DNA-based metabarcoding is increasingly used to monitor diversity of soil organisms. Few studies have compared this method with conventional methods. The aim of the study was to compare metabarcoding based on the mitochondrial 16S rRNA region with conventional morphology-based identification, where metabarcoding captured more species (eight) than identified by conventional method (five). Additionally, we tested the effects of two commercial DNA extraction kits: PowerSoil based on 0.25 g of soil and PowerMax based on 10 g of soil (both from Qiagen), where the PowerMax covered higher richness than PowerSoil.
Original language | English |
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Article number | 104798 |
Journal | Applied Soil Ecology |
Volume | 185 |
Number of pages | 4 |
ISSN | 0929-1393 |
DOIs | |
Publication status | Published - May 2023 |
Keywords
- Community barcoding
- DNA extraction
- Environmental DNA
- Mitochondrial 16S rRNA
- Soil
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Advice on soil invertebrates and their ecology, diversity and ecotoxicology
Krogh, P. H. (PI)
01/01/1997 → …
Project: Consultancy
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eDNA Center - Applied molecular tools for environmental monitoring and research
Winding, A. (PI), Sapkota, R. (PI), Zervas, T. (PI), Hendriksen, N. B. (PI), Stougaard, P. (PI), Thøgersen, M. S. (PI), Barbosa Anesio, A. M. (PI), Zeng, Y. (PI), Johansen, A. (PI), Jacobsen, C. S. (PI), Ørby, P. V. (PI), Stæhr, P. A. (PI), Hansen, J. L. S. (PI), Jakobsen, H. (PI), Krause-Jensen, D. (PI), Krogh, P. H. (PI), Schmidt, N. M. (PI), Scheel, M. (PI), Andersen, L. W. (PI), Nicolaisen, M. (PI), Madsen, M. V. (PI), Santos, S. (PI), Kudjordjie, E. N. (PI) & Sikder, M. M. (PI)
01/01/2020 → 30/11/2023
Project: Research
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Climate and Environmental Effects of Nitrification Inhibitors
Winding, A. (Project coordinator), Krogh, P. H. (PI), Bruun, S. (PI), Petersen, S. O. (PI), Sapkota, R. (PI), Buivydaite, Z. (PI), Thane, T. (PI) & Bruus, M. (PI)
01/09/2019 → 28/02/2024
Project: Research