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Common Effects on Follicular Thyroid Cancer Cells Exerted by Simulated Microgravity

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This study focuses on gravity-sensitive proteins of two human follicular cancer cell lines (ML-1; RO82-W-1), which were exposed to simulated microgravity (s-μg) on two different machines. Changes in protein cytoskeletal structure, growth patterns and protein expression in response to s-μg were investigated. After a three-day (3d) or seven-day (7d) culture grown in s-μg, we found both cell types growing three-dimensionally within multicellular spheroids (MCS) and also cells remaining adherent (AD) to the culture flask, while 1g- control cultures only formed adherent monolayers RO82-W-1 cells released significantly increased amounts of IL-6 and IL-8 when cultured on the RPM, but not on the clinostat. Culture of RO82-W-1 cells on the RPM or the CLINO completely blunted the release of MCP-1. ML-1 cells grown in s-μg released significantly elevated amounts of IL-6 and MCP-1 into the supernatant when compared to controls. After 3d of s-μg, an accumulation of F-actin around the cellular membrane was detectable in AD cells of both cell lines.
Original languageEnglish
Publication year19 Sep 2015
Publication statusPublished - 19 Sep 2015

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