Combination of Rhamnolipid and Chitosan in Nanoparticles Boosts Their Antimicrobial Efficacy

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Combination of Rhamnolipid and Chitosan in Nanoparticles Boosts Their Antimicrobial Efficacy. / Marangon, Crisiane A.; Martins, Virginia C.A.; Ling, Ma H.; Melo, Cristiane C.; Plepis, Ana Maria G.; Meyer, Rikke L.; Nitschke, Marcia.

In: ACS Applied Materials and Interfaces, Vol. 12, No. 5, 2020, p. 5488-5499.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Marangon, CA, Martins, VCA, Ling, MH, Melo, CC, Plepis, AMG, Meyer, RL & Nitschke, M 2020, 'Combination of Rhamnolipid and Chitosan in Nanoparticles Boosts Their Antimicrobial Efficacy', ACS Applied Materials and Interfaces, vol. 12, no. 5, pp. 5488-5499. https://doi.org/10.1021/acsami.9b19253

APA

Marangon, C. A., Martins, V. C. A., Ling, M. H., Melo, C. C., Plepis, A. M. G., Meyer, R. L., & Nitschke, M. (2020). Combination of Rhamnolipid and Chitosan in Nanoparticles Boosts Their Antimicrobial Efficacy. ACS Applied Materials and Interfaces, 12(5), 5488-5499. https://doi.org/10.1021/acsami.9b19253

CBE

Marangon CA, Martins VCA, Ling MH, Melo CC, Plepis AMG, Meyer RL, Nitschke M. 2020. Combination of Rhamnolipid and Chitosan in Nanoparticles Boosts Their Antimicrobial Efficacy. ACS Applied Materials and Interfaces. 12(5):5488-5499. https://doi.org/10.1021/acsami.9b19253

MLA

Marangon, Crisiane A. et al. "Combination of Rhamnolipid and Chitosan in Nanoparticles Boosts Their Antimicrobial Efficacy". ACS Applied Materials and Interfaces. 2020, 12(5). 5488-5499. https://doi.org/10.1021/acsami.9b19253

Vancouver

Marangon CA, Martins VCA, Ling MH, Melo CC, Plepis AMG, Meyer RL et al. Combination of Rhamnolipid and Chitosan in Nanoparticles Boosts Their Antimicrobial Efficacy. ACS Applied Materials and Interfaces. 2020;12(5):5488-5499. https://doi.org/10.1021/acsami.9b19253

Author

Marangon, Crisiane A. ; Martins, Virginia C.A. ; Ling, Ma H. ; Melo, Cristiane C. ; Plepis, Ana Maria G. ; Meyer, Rikke L. ; Nitschke, Marcia. / Combination of Rhamnolipid and Chitosan in Nanoparticles Boosts Their Antimicrobial Efficacy. In: ACS Applied Materials and Interfaces. 2020 ; Vol. 12, No. 5. pp. 5488-5499.

Bibtex

@article{d3692f0efc4241658dc0d5a5b090ca0b,
title = "Combination of Rhamnolipid and Chitosan in Nanoparticles Boosts Their Antimicrobial Efficacy",
abstract = "Nanomaterials have emerged as antimicrobial agents due to their unique physical and chemical properties. The development of nanoparticles (NPs) composed of natural biopolymers and biosurfactants have sparked interest, as they can be obtained without the use of complex chemical synthesis and toxic materials. In this study, we develop antimicrobial nanoparticles combining the biopolymer chitosan with the biosurfactant rhamnolipid. Addition of rhamnolipid reduced the size and polydispersity index of chitosan nanoparticles showing a more positive surface charge with improved stability, suggesting that chitosan-free amino groups are predominantly present on the surface of nanoparticles. Antimicrobial activity of chitosan/rhamnolipid nanoparticles (C/RL-NPs) against Staphylococcus strains surpassed that of either single rhamnolipid or chitosan, both in planktonic bacteria and biofilms. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of C/RL-NPs were determined considering the concentration of each individual molecule in NPs. MIC values of 14/19 μg mL-1 and MBC of 29/37 μg mL-1 were observed for S. aureus DSM 1104 and MIC and MBC of 29/37 and 58/75 μg mL-1 were observed against S. aureus ATCC 29213, respectively. For S. epidermidis, MIC and MBC of 7/9 and 14/19 μg mL-1 were noticed. Chitosan and chitosan nanoparticles eliminate the bacteria present in the upper parts of biofilms, while C/RL-NPs were more effective, eradicating most sessile bacteria and reducing the number of viable cells below the detection limit, when NPs concentration of 58/75 μg mL-1 was applied for both S. aureus DSM 1104 and S. epidermidis biofilms. The improved antibacterial efficacy of C/RL-NPs was linked to the increased local delivery of chitosan and rhamnolipid at the cell surface and, consequently, to their targets in Gram-positive bacteria. The combination of chitosan and rhamnolipid offers a promising strategy to the design of novel nanoparticles with low cytotoxicity, which can be exploited in pharmaceutical and food industries.",
keywords = "antimicrobial activity, biosurfactant, chitosan, nanoparticle, rhamnolipid",
author = "Marangon, {Crisiane A.} and Martins, {Virginia C.A.} and Ling, {Ma H.} and Melo, {Cristiane C.} and Plepis, {Ana Maria G.} and Meyer, {Rikke L.} and Marcia Nitschke",
year = "2020",
doi = "10.1021/acsami.9b19253",
language = "English",
volume = "12",
pages = "5488--5499",
journal = "A C S Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "5",

}

RIS

TY - JOUR

T1 - Combination of Rhamnolipid and Chitosan in Nanoparticles Boosts Their Antimicrobial Efficacy

AU - Marangon, Crisiane A.

AU - Martins, Virginia C.A.

AU - Ling, Ma H.

AU - Melo, Cristiane C.

AU - Plepis, Ana Maria G.

AU - Meyer, Rikke L.

AU - Nitschke, Marcia

PY - 2020

Y1 - 2020

N2 - Nanomaterials have emerged as antimicrobial agents due to their unique physical and chemical properties. The development of nanoparticles (NPs) composed of natural biopolymers and biosurfactants have sparked interest, as they can be obtained without the use of complex chemical synthesis and toxic materials. In this study, we develop antimicrobial nanoparticles combining the biopolymer chitosan with the biosurfactant rhamnolipid. Addition of rhamnolipid reduced the size and polydispersity index of chitosan nanoparticles showing a more positive surface charge with improved stability, suggesting that chitosan-free amino groups are predominantly present on the surface of nanoparticles. Antimicrobial activity of chitosan/rhamnolipid nanoparticles (C/RL-NPs) against Staphylococcus strains surpassed that of either single rhamnolipid or chitosan, both in planktonic bacteria and biofilms. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of C/RL-NPs were determined considering the concentration of each individual molecule in NPs. MIC values of 14/19 μg mL-1 and MBC of 29/37 μg mL-1 were observed for S. aureus DSM 1104 and MIC and MBC of 29/37 and 58/75 μg mL-1 were observed against S. aureus ATCC 29213, respectively. For S. epidermidis, MIC and MBC of 7/9 and 14/19 μg mL-1 were noticed. Chitosan and chitosan nanoparticles eliminate the bacteria present in the upper parts of biofilms, while C/RL-NPs were more effective, eradicating most sessile bacteria and reducing the number of viable cells below the detection limit, when NPs concentration of 58/75 μg mL-1 was applied for both S. aureus DSM 1104 and S. epidermidis biofilms. The improved antibacterial efficacy of C/RL-NPs was linked to the increased local delivery of chitosan and rhamnolipid at the cell surface and, consequently, to their targets in Gram-positive bacteria. The combination of chitosan and rhamnolipid offers a promising strategy to the design of novel nanoparticles with low cytotoxicity, which can be exploited in pharmaceutical and food industries.

AB - Nanomaterials have emerged as antimicrobial agents due to their unique physical and chemical properties. The development of nanoparticles (NPs) composed of natural biopolymers and biosurfactants have sparked interest, as they can be obtained without the use of complex chemical synthesis and toxic materials. In this study, we develop antimicrobial nanoparticles combining the biopolymer chitosan with the biosurfactant rhamnolipid. Addition of rhamnolipid reduced the size and polydispersity index of chitosan nanoparticles showing a more positive surface charge with improved stability, suggesting that chitosan-free amino groups are predominantly present on the surface of nanoparticles. Antimicrobial activity of chitosan/rhamnolipid nanoparticles (C/RL-NPs) against Staphylococcus strains surpassed that of either single rhamnolipid or chitosan, both in planktonic bacteria and biofilms. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of C/RL-NPs were determined considering the concentration of each individual molecule in NPs. MIC values of 14/19 μg mL-1 and MBC of 29/37 μg mL-1 were observed for S. aureus DSM 1104 and MIC and MBC of 29/37 and 58/75 μg mL-1 were observed against S. aureus ATCC 29213, respectively. For S. epidermidis, MIC and MBC of 7/9 and 14/19 μg mL-1 were noticed. Chitosan and chitosan nanoparticles eliminate the bacteria present in the upper parts of biofilms, while C/RL-NPs were more effective, eradicating most sessile bacteria and reducing the number of viable cells below the detection limit, when NPs concentration of 58/75 μg mL-1 was applied for both S. aureus DSM 1104 and S. epidermidis biofilms. The improved antibacterial efficacy of C/RL-NPs was linked to the increased local delivery of chitosan and rhamnolipid at the cell surface and, consequently, to their targets in Gram-positive bacteria. The combination of chitosan and rhamnolipid offers a promising strategy to the design of novel nanoparticles with low cytotoxicity, which can be exploited in pharmaceutical and food industries.

KW - antimicrobial activity

KW - biosurfactant

KW - chitosan

KW - nanoparticle

KW - rhamnolipid

UR - http://www.scopus.com/inward/record.url?scp=85078951703&partnerID=8YFLogxK

U2 - 10.1021/acsami.9b19253

DO - 10.1021/acsami.9b19253

M3 - Journal article

C2 - 31927982

AN - SCOPUS:85078951703

VL - 12

SP - 5488

EP - 5499

JO - A C S Applied Materials and Interfaces

JF - A C S Applied Materials and Interfaces

SN - 1944-8244

IS - 5

ER -