Cloning, sequencing and variability analysis of the gap gene from Mycoplasma hominis

Tina Mygind, Iben Søgaard Jacobsen, Renata Melkova, Thomas Boesen, Svend Birkelund, Gunna Christiansen

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review


    The gap gene encodes the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The gene was cloned and sequenced from the Mycoplasma hominis type strain PG21(T). The intraspecies variability was investigated by inspection of restriction fragment length polymorphism (RFLP) patterns after polymerase chain reaction (PCR) amplification of the gap gene from 15 strains and furthermore by sequencing of part of the gene in eight strains. The M. hominis gap gene was found to vary more than the Escherichia coli counterpart, but the variation at nucleotide level gave rise to only a few amino acid substitutions. To verify that the gene was expressed in M. hominis, a polyclonal antibody was produced and tested against whole cell protein from 15 strains. The enzyme was expressed in all strains investigated as a 36-kDa protein. All strains except type strain PG21(T) showed reaction to a 104-kDa band in addition to the expected 36-kDa band. The protein reacting at 104 kDa is a M. hominis protein with either an epitope similar to one on GAPDH, or it is an immunoglobulin binding protein
    Original languageEnglish
    JournalF E M S Microbiology Letters
    Pages (from-to)15-21
    Number of pages7
    Publication statusPublished - 2000


    Dive into the research topics of 'Cloning, sequencing and variability analysis of the gap gene from Mycoplasma hominis'. Together they form a unique fingerprint.

    Cite this