Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

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  • Munsoor Ali Hanifa, Aalborg University, Denmark
  • Raluca G. Maltesen
  • Bodil S. Rasmussen
  • ,
  • Katrine B. Buggeskov, Copenhagen University Hospital
  • ,
  • Hanne Berg Ravn, Copenhagen University Hospital, Denmark
  • Martin Skott
  • Søren Nielsen, Meta-IQ, Denmark
  • Jørgen Frøkiær
  • Troels Ring, University of Pittsburgh
  • ,
  • Reinhard Wimmer, Aalborg University
© 2019, Springer Science+Business Media, LLC, part of Springer Nature. Background: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities. Aims: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies. Methods: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated. Results: Integral changes were generally small, but serum citrate signals decreased significantly in some samples. Conclusions: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.
Original languageEnglish
Article number7
JournalMetabolomics
Volume16
Issue1
Number of pages7
ISSN1573-3882
DOIs
Publication statusPublished - Jan 2020

    Research areas

  • Metabolomics, NMR, Plasma, Reproducibility, Serum

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