Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Munsoor Ali Hanifa, Aalborg University, Denmark
  • Raluca G. Maltesen, Aalborg University
  • ,
  • Bodil S. Rasmussen
  • ,
  • Katrine B. Buggeskov, University of Copenhagen
  • ,
  • Hanne Berg Ravn, University of Copenhagen, Denmark
  • Martin Skott
  • Søren Nielsen, Meta-IQ, Denmark
  • Jørgen Frøkiær
  • Troels Ring, University of Pittsburgh
  • ,
  • Reinhard Wimmer, Aalborg University
© 2019, Springer Science+Business Media, LLC, part of Springer Nature. Background: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities. Aims: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies. Methods: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated. Results: Integral changes were generally small, but serum citrate signals decreased significantly in some samples. Conclusions: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.
Original languageEnglish
Article number7
Number of pages7
Publication statusPublished - Jan 2020

    Research areas

  • Metabolomics, NMR, Plasma, Reproducibility, Serum

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