Cisgenic Barley with Improved Phytase Activity

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Cisgenic Barley with Improved Phytase Activity. / Holme, Inger; Dionisio, Giuseppe; Brinch-Pedersen, Henrik; Vincze, Éva; Holm, Preben Bach.

In: In Vitro Cellular & Developmental Biology - Animal, Vol. 46, No. Supplement 1, 2010, p. 187.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperConference abstract in journalResearchpeer-review

Harvard

Holme, I, Dionisio, G, Brinch-Pedersen, H, Vincze, É & Holm, PB 2010, 'Cisgenic Barley with Improved Phytase Activity', In Vitro Cellular & Developmental Biology - Animal, vol. 46, no. Supplement 1, pp. 187. https://doi.org/10.1007/s11626-010-9339-6

APA

Holme, I., Dionisio, G., Brinch-Pedersen, H., Vincze, É., & Holm, P. B. (2010). Cisgenic Barley with Improved Phytase Activity. In Vitro Cellular & Developmental Biology - Animal, 46(Supplement 1), 187. https://doi.org/10.1007/s11626-010-9339-6

CBE

Holme I, Dionisio G, Brinch-Pedersen H, Vincze É, Holm PB. 2010. Cisgenic Barley with Improved Phytase Activity. In Vitro Cellular & Developmental Biology - Animal. 46(Supplement 1):187. https://doi.org/10.1007/s11626-010-9339-6

MLA

Holme, Inger et al. "Cisgenic Barley with Improved Phytase Activity". In Vitro Cellular & Developmental Biology - Animal. 2010, 46(Supplement 1). 187. https://doi.org/10.1007/s11626-010-9339-6

Vancouver

Holme I, Dionisio G, Brinch-Pedersen H, Vincze É, Holm PB. Cisgenic Barley with Improved Phytase Activity. In Vitro Cellular & Developmental Biology - Animal. 2010;46(Supplement 1):187. https://doi.org/10.1007/s11626-010-9339-6

Author

Holme, Inger ; Dionisio, Giuseppe ; Brinch-Pedersen, Henrik ; Vincze, Éva ; Holm, Preben Bach. / Cisgenic Barley with Improved Phytase Activity. In: In Vitro Cellular & Developmental Biology - Animal. 2010 ; Vol. 46, No. Supplement 1. pp. 187.

Bibtex

@article{46e824cc63c24c2a9e048b72902f6f0d,
title = "Cisgenic Barley with Improved Phytase Activity",
abstract = "Genetic transformation is currently met with substantial skepticism among the general public in Europe and consequently also by the growers, the agro-industry, and the retailers. One major concern is the mingling of genetic material between species. In the light of this, we have initiated a project based on the cisgenesis concept. In contrast to transgenesis, cisgenesis implies that the plant is transformed only with its own or very closely related genetic material. Furthermore, all “helper” genes and gene sequences of foreign nature are removed from the transformed plant lines. Cisgenic crops are accordingly very similar to those generated by conventional breeding. The cisgenesis concept allows for the introduction of extra gene copies of a particular gene to accentuate the trait. We are using a barley purple acid phosphatase expressed during grain filling as candidate gene for cisgenesis. A genomic barley lambda library has been used to isolate the genomic clone of this phytase including 2.3 kb of the promoter region and 600 bp of the terminator region. The clone has been inserted into a cisgenic Agrobacterium vector where both the gene of interest and the selection gene are flanked by their own T-DNA borders in order to promote integration of the two genes at unlinked places in the plant genome. Transformed T0 plants show increases in the phytase activity of mature seeds from 1,400 in wild type to 8,950 FTU/kg in T0 plants. T1 plants of each transformant are currently screened with PCR for extra copies of the genomic phytase gene and the selection gene to identify segregation between the two genes. Presently, we have identified two cisgenic T1 plants without vector backbone and selection gene but with an extra copy of the genomic phytase gene.",
author = "Inger Holme and Giuseppe Dionisio and Henrik Brinch-Pedersen and {\'E}va Vincze and Holm, {Preben Bach}",
year = "2010",
doi = "10.1007/s11626-010-9339-6",
language = "English",
volume = "46",
pages = "187",
journal = "In Vitro Cellular & Developmental Biology - Animal",
issn = "1071-2690",
publisher = "Springer",
number = "Supplement 1",

}

RIS

TY - ABST

T1 - Cisgenic Barley with Improved Phytase Activity

AU - Holme, Inger

AU - Dionisio, Giuseppe

AU - Brinch-Pedersen, Henrik

AU - Vincze, Éva

AU - Holm, Preben Bach

PY - 2010

Y1 - 2010

N2 - Genetic transformation is currently met with substantial skepticism among the general public in Europe and consequently also by the growers, the agro-industry, and the retailers. One major concern is the mingling of genetic material between species. In the light of this, we have initiated a project based on the cisgenesis concept. In contrast to transgenesis, cisgenesis implies that the plant is transformed only with its own or very closely related genetic material. Furthermore, all “helper” genes and gene sequences of foreign nature are removed from the transformed plant lines. Cisgenic crops are accordingly very similar to those generated by conventional breeding. The cisgenesis concept allows for the introduction of extra gene copies of a particular gene to accentuate the trait. We are using a barley purple acid phosphatase expressed during grain filling as candidate gene for cisgenesis. A genomic barley lambda library has been used to isolate the genomic clone of this phytase including 2.3 kb of the promoter region and 600 bp of the terminator region. The clone has been inserted into a cisgenic Agrobacterium vector where both the gene of interest and the selection gene are flanked by their own T-DNA borders in order to promote integration of the two genes at unlinked places in the plant genome. Transformed T0 plants show increases in the phytase activity of mature seeds from 1,400 in wild type to 8,950 FTU/kg in T0 plants. T1 plants of each transformant are currently screened with PCR for extra copies of the genomic phytase gene and the selection gene to identify segregation between the two genes. Presently, we have identified two cisgenic T1 plants without vector backbone and selection gene but with an extra copy of the genomic phytase gene.

AB - Genetic transformation is currently met with substantial skepticism among the general public in Europe and consequently also by the growers, the agro-industry, and the retailers. One major concern is the mingling of genetic material between species. In the light of this, we have initiated a project based on the cisgenesis concept. In contrast to transgenesis, cisgenesis implies that the plant is transformed only with its own or very closely related genetic material. Furthermore, all “helper” genes and gene sequences of foreign nature are removed from the transformed plant lines. Cisgenic crops are accordingly very similar to those generated by conventional breeding. The cisgenesis concept allows for the introduction of extra gene copies of a particular gene to accentuate the trait. We are using a barley purple acid phosphatase expressed during grain filling as candidate gene for cisgenesis. A genomic barley lambda library has been used to isolate the genomic clone of this phytase including 2.3 kb of the promoter region and 600 bp of the terminator region. The clone has been inserted into a cisgenic Agrobacterium vector where both the gene of interest and the selection gene are flanked by their own T-DNA borders in order to promote integration of the two genes at unlinked places in the plant genome. Transformed T0 plants show increases in the phytase activity of mature seeds from 1,400 in wild type to 8,950 FTU/kg in T0 plants. T1 plants of each transformant are currently screened with PCR for extra copies of the genomic phytase gene and the selection gene to identify segregation between the two genes. Presently, we have identified two cisgenic T1 plants without vector backbone and selection gene but with an extra copy of the genomic phytase gene.

U2 - 10.1007/s11626-010-9339-6

DO - 10.1007/s11626-010-9339-6

M3 - Conference abstract in journal

VL - 46

SP - 187

JO - In Vitro Cellular & Developmental Biology - Animal

JF - In Vitro Cellular & Developmental Biology - Animal

SN - 1071-2690

IS - Supplement 1

ER -