The addition of non-pollen palynomorphs (NPPs) to pollen analytical studies has improved the interpretational frame, especially concerning the local regime in anthropogenically disturbed environments. Using advanced ordination techniques this paper explores the variation of NPP-assemblages and the indicative value of individual NPP-types by comparison to independent classical proxies. Sediment samples from a forest hollow at Tårup Lund, Denmark, covering the last 5,500 years, were prepared for NPP-analysis using a non-aggressive procedure. Correlations between non-pollen palynomorphs and sediment, pollen and macrofossil inferred environmental explanatory variables were studied based on a constant counting sum of NPPs. This approach identified main drivers influencing NPP assemblage composition and indicated that a change in these drivers occurred at the onset of the Pre-Roman Iron Age. Twelve known and 12 specific, but so far unknown, NPPs correlating with the environmental variables were identified and their possible indicator value presented. The mild preparation procedure resulted in a very large dataset allowing for sub-assemblages to be explored separately. This approach indicated the potential for identifying further environmental indicators among these groups.