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Circulating human B and plasma cells. Age-associated changes in counts and detailed characterization of circulating normal CD138- and CD138+ plasma cells

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  • Anouk Caraux, Denmark
  • Bernard Klein, Denmark
  • Bruno Paiva, Denmark
  • Caroline Bret, Denmark
  • Alexander Schmitz, Denmark
  • Gwenny M Fuhler, Denmark
  • Nico A Bos, Denmark
  • Hans E Johnsen, Denmark
  • Alberto Orfao, Denmark
  • Martin Perez-Andres, Denmark
  • for the Myeloma Stem Cell Network (MSCNET)
  • Hæmatologisk Afdeling, Aalborg Sygehus
  • Department of Molecular Biology
Generation of B and plasma cells (PC) involves several organs with a necessary cell trafficking between them. A detailed phenotypic characterization of four circulating B-cell subsets - immature-, naïve-, memory- B-lymphocytes and PC - of 106 healthy adults was realized by multiparametric flow cytometry. We show that CD10, CD27 and CD38 is the minimal combination of subsetting markers allowing unequivocal identification of immature (CD10(+)CD27(-)CD38(+), 6+/-6 cells/mul), naïve (CD10(-)CD27(-)CD38(-), 125+/-90 cells/mul), memory B-lymphocytes (CD10(-)CD27(+)CD38(-), 58+/-42 cells/mul), and PC (CD10(-)CD27(++)CD38(++), 2.1+/-2.1 cells/mul) within circulating CD19(+) cells. From these four subsets, only memory B-lymphocytes and PC decreased with age, both in relative and absolute counts. Circulating PC split into CD138(-) (57%+/-12%) and CD138(+) (43%+/-12%) cells, the latter displaying a more mature phenotypic profile: absence of surface immunoglobulin, lower CD45 positivity and higher amounts of cytoplasmic immunoglobulin, CD38 and CD27. Unlike B-lymphocytes, both populations of PC are KI-67(+) and show weak CXCR4 expression.
Original languageEnglish
Pages (from-to)1016-20
Number of pages5
Publication statusPublished - 2010

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