Circulating Extracellular Vesicle‐associated CD163 and CD206 in Multiple Myeloma

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Objectives Extracellular vesicles (EVs) are important for intercellular signalling in cancer. Tumour-associated macrophages, expressing the haemoglobin-haptoglobin and mannose receptors CD163 and CD206, are crucial for cancer progression. We recently identified CD163 on EVs in the circulation as a fraction of total soluble CD163 (sCD163). Here, we investigated the presence of CD163 and CD206-positive EVs (EV-CD163, EV-CD206) in patients with multiple myeloma (MM).

Methods We enrolled patients with MM (n = 32), monoclonal gammopathy of undetermined significance (MGUS) (n = 8) and healthy donors (n = 16). Plasma protein levels were determined by ELISA before and after vesicle precipitation. Monocytes were examined by flow cytometry, and leucocyte CD163 mRNA by qPCR.

Results Fractions of EV-CD163 and EV-CD206 were significantly elevated in patients with newly diagnosed MM (median = 39.8%, 76.5%, respectively) compared to patients with relapse (15.6%, P = .02, 42.5%, P = .003), remission (16.9%, P <.0001, 25.2%, P <.0001), MGUS (17.8%, P <.01, 33.1%, P = .0005) and healthy donors (14.8%, P <.0001, 35.5%, P <.0001). Whole blood CD163 mRNA did not vary between the groups. The intermediate monocyte subset showed a higher CD163 expression in newly diagnosed patients.

Conclusions Our results indicate that macrophage-derived EVs may play a role in the late phase of malignant progression of MM, and encourage further EV investigations in functional experiments.

Original languageEnglish
JournalEuropean Journal of Haematology
Volume104
Issue5
Pages (from-to)409-419
Number of pages11
ISSN0902-4441
DOIs
Publication statusPublished - 2020

    Research areas

  • ACTIVATION, CD163, CD206, FLOW-CYTOMETRY, HUMAN MONOCYTES, IDENTIFICATION, INDUCTION, MANNOSE RECEPTOR, MICROENVIRONMENT, MONOCLONAL GAMMOPATHY, PROGRESSION, TUMOR-ASSOCIATED MACROPHAGES, extracellular vesicles, molecular cytogenetics, multiple myeloma, plasma cell neoplasms, tumour-associated macrophage

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