Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth

Ursula Mittnacht, Hanna Hartmann, San Hein, Hugo Oliveira, Mingdong Dong, Ana P Pêgo, Jørgen Kjems, Kenneth A Howard, Burkhard Schlosshauer

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review


    Microstructured 20 μm thick polymer filaments used as nerve implants were loaded with chitosan/siRNA nanoparticles to promote nerve regeneration and ensure local delivery of nanotherapeutics. The stable nanoparticles were rapidly internalized by cells and did not affect cell viability. Target mRNA was successfully reduced by 65-75% and neurite outgrowth was enhanced even in an inhibitory environment. This work, thus, supports the application of nanobiofunctionalized implants as a novel approach for spinal cord and nerve repair.
    Original languageEnglish
    JournalNano Letters
    Pages (from-to)3933-9
    Number of pages7
    Publication statusPublished - 13 Oct 2010


    • Animals
    • Cell Line
    • Chitosan
    • Nanoparticles
    • Nerve Regeneration
    • Neurites
    • Neurons
    • Prostheses and Implants
    • RNA, Small Interfering
    • Transfection
    • rhoA GTP-Binding Protein


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