Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth

Ursula Mittnacht, Hanna Hartmann, San Hein, Hugo Oliveira, Mingdong Dong, Ana P Pêgo, Jørgen Kjems, Kenneth A Howard, Burkhard Schlosshauer

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    Abstract

    Microstructured 20 μm thick polymer filaments used as nerve implants were loaded with chitosan/siRNA nanoparticles to promote nerve regeneration and ensure local delivery of nanotherapeutics. The stable nanoparticles were rapidly internalized by cells and did not affect cell viability. Target mRNA was successfully reduced by 65-75% and neurite outgrowth was enhanced even in an inhibitory environment. This work, thus, supports the application of nanobiofunctionalized implants as a novel approach for spinal cord and nerve repair.
    Original languageEnglish
    JournalNano Letters
    Volume10
    Issue10
    Pages (from-to)3933-9
    Number of pages7
    ISSN1530-6984
    DOIs
    Publication statusPublished - 13 Oct 2010

    Keywords

    • Animals
    • Cell Line
    • Chitosan
    • Nanoparticles
    • Nerve Regeneration
    • Neurites
    • Neurons
    • Prostheses and Implants
    • RNA, Small Interfering
    • Transfection
    • rhoA GTP-Binding Protein

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