Chitosan oligosaccharides-triggered innate immunity contributes to oilseed rape resistance against Sclerotinia sclerotiorum

Heng Yin; Li Yan; Zhang HongYan; Wang WenXia; Lu Hang; Kai Grevsen; Zhao XiaoMing; Du YuGuang

doi:10.1086/669721

Chitosan oligosaccharides-triggered innate immunity contributes to oilseed rape resistance against Sclerotinia sclerotiorum

Heng Yin, Li Yan, Zhang HongYan, Wang WenXia, Lu Hang, Kai Grevsen, Zhao XiaoMing, Du YuGuang

Department of Food Science - Differentiated & Biofunctional Foods

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

60 Citations (Scopus)

Abstract

Chitosan oligosaccharides (collectively, oligochitosan, or COS) are considered to be potent plant immunity elicitors. In this article, the induction of resistance to Sclerotinia sclerotiorum in Brassica napus L. var. Huyou 15 by COS is studied. Even though COS (50 mg mL1) did not affect radial growth of this pathogen in vitro, it reduced the disease symptoms in vivo relative to control plants. The disease control rates were 25.8%, 41.4%, 57.1%, 68.7%, and 48.8% with COS pretreatment 0, 24, 48, 72, and 96 h before S. sclerotiorum
inoculation, respectively. Specific binding of COS to B. napus epidermis cells was validated by competition experiments. Simultaneously, it was observed that COS induced bursts of cytosolic Ca2, nitric oxide (NO), and hydrogen peroxide (H2O2). NO and H2O2 inhibitors were used to prove the interaction between NO and H2O2. Furthermore, treatments of B. napus with NO and H2O2 inhibitors reduced the induction effect of the jasmonic acid–ethylene (JA/ET) signaling marker BnPDF1.2 by COS, indicating that NO and H2O2 participate in COS-induced JA/ET signaling. In conclusion, this article provides a comprehensive study of the effect and mechanism of COS-induced resistance to S. sclerotiorum in B. napus.

Original language	English
Journal	International Journal of Plant Sciences
Volume	174
Issue	4
Pages (from-to)	722-732
Number of pages	11
ISSN	1058-5893
DOIs	https://doi.org/10.1086/669721
Publication status	Published - 2013

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@article{3b9451ebac1c4dd29ba7df7be7a4aa1d,

title = "Chitosan oligosaccharides-triggered innate immunity contributes to oilseed rape resistance against Sclerotinia sclerotiorum",

abstract = "Chitosan oligosaccharides (collectively, oligochitosan, or COS) are considered to be potent plant immunity elicitors. In this article, the induction of resistance to Sclerotinia sclerotiorum in Brassica napus L. var. Huyou 15 by COS is studied. Even though COS (50 mg mL1) did not affect radial growth of this pathogen in vitro, it reduced the disease symptoms in vivo relative to control plants. The disease control rates were 25.8\%, 41.4\%, 57.1\%, 68.7\%, and 48.8\% with COS pretreatment 0, 24, 48, 72, and 96 h before S. sclerotiorum inoculation, respectively. Specific binding of COS to B. napus epidermis cells was validated by competition experiments. Simultaneously, it was observed that COS induced bursts of cytosolic Ca2, nitric oxide (NO), and hydrogen peroxide (H2O2). NO and H2O2 inhibitors were used to prove the interaction between NO and H2O2. Furthermore, treatments of B. napus with NO and H2O2 inhibitors reduced the induction effect of the jasmonic acid–ethylene (JA/ET) signaling marker BnPDF1.2 by COS, indicating that NO and H2O2 participate in COS-induced JA/ET signaling. In conclusion, this article provides a comprehensive study of the effect and mechanism of COS-induced resistance to S. sclerotiorum in B. napus.",

keywords = "chitosan oligosaccharide, brassica napus, Schlerotinia sclerotiorum, plant immunity, elicitor",

author = "Heng Yin and Li Yan and Zhang HongYan and Wang WenXia and Lu Hang and Kai Grevsen and Zhao XiaoMing and Du YuGuang",

year = "2013",

doi = "10.1086/669721",

language = "English",

volume = "174",

pages = "722--732 ",

journal = "International Journal of Plant Sciences",

issn = "1058-5893",

publisher = "University of Chicago Press",

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}

Chitosan oligosaccharides-triggered innate immunity contributes to oilseed rape resistance against Sclerotinia sclerotiorum. / Yin, Heng; Yan, Li; HongYan, Zhang et al.
In: International Journal of Plant Sciences, Vol. 174, No. 4, 2013, p. 722-732 .

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Chitosan oligosaccharides-triggered innate immunity contributes to oilseed rape resistance against Sclerotinia sclerotiorum

AU - Yin, Heng

AU - Yan, Li

AU - HongYan, Zhang

AU - WenXia, Wang

AU - Hang, Lu

AU - Grevsen, Kai

AU - XiaoMing, Zhao

AU - YuGuang, Du

PY - 2013

Y1 - 2013

N2 - Chitosan oligosaccharides (collectively, oligochitosan, or COS) are considered to be potent plant immunity elicitors. In this article, the induction of resistance to Sclerotinia sclerotiorum in Brassica napus L. var. Huyou 15 by COS is studied. Even though COS (50 mg mL1) did not affect radial growth of this pathogen in vitro, it reduced the disease symptoms in vivo relative to control plants. The disease control rates were 25.8%, 41.4%, 57.1%, 68.7%, and 48.8% with COS pretreatment 0, 24, 48, 72, and 96 h before S. sclerotiorum inoculation, respectively. Specific binding of COS to B. napus epidermis cells was validated by competition experiments. Simultaneously, it was observed that COS induced bursts of cytosolic Ca2, nitric oxide (NO), and hydrogen peroxide (H2O2). NO and H2O2 inhibitors were used to prove the interaction between NO and H2O2. Furthermore, treatments of B. napus with NO and H2O2 inhibitors reduced the induction effect of the jasmonic acid–ethylene (JA/ET) signaling marker BnPDF1.2 by COS, indicating that NO and H2O2 participate in COS-induced JA/ET signaling. In conclusion, this article provides a comprehensive study of the effect and mechanism of COS-induced resistance to S. sclerotiorum in B. napus.

AB - Chitosan oligosaccharides (collectively, oligochitosan, or COS) are considered to be potent plant immunity elicitors. In this article, the induction of resistance to Sclerotinia sclerotiorum in Brassica napus L. var. Huyou 15 by COS is studied. Even though COS (50 mg mL1) did not affect radial growth of this pathogen in vitro, it reduced the disease symptoms in vivo relative to control plants. The disease control rates were 25.8%, 41.4%, 57.1%, 68.7%, and 48.8% with COS pretreatment 0, 24, 48, 72, and 96 h before S. sclerotiorum inoculation, respectively. Specific binding of COS to B. napus epidermis cells was validated by competition experiments. Simultaneously, it was observed that COS induced bursts of cytosolic Ca2, nitric oxide (NO), and hydrogen peroxide (H2O2). NO and H2O2 inhibitors were used to prove the interaction between NO and H2O2. Furthermore, treatments of B. napus with NO and H2O2 inhibitors reduced the induction effect of the jasmonic acid–ethylene (JA/ET) signaling marker BnPDF1.2 by COS, indicating that NO and H2O2 participate in COS-induced JA/ET signaling. In conclusion, this article provides a comprehensive study of the effect and mechanism of COS-induced resistance to S. sclerotiorum in B. napus.

KW - chitosan oligosaccharide

KW - brassica napus

KW - Schlerotinia sclerotiorum

KW - plant immunity

KW - elicitor

U2 - 10.1086/669721

DO - 10.1086/669721

M3 - Journal article

SN - 1058-5893

VL - 174

SP - 722

EP - 732

JO - International Journal of Plant Sciences

JF - International Journal of Plant Sciences

IS - 4

ER -