Chitosan-coated Poly(lactic-co-glycolic acid) Perfluorooctyl Bromide Nanoparticles for Cell Labeling in 19F Magnetic Resonance Imaging

Hieu Vu Quang; Mads Sloth Vinding; Dan Xia; Thomas Nielsen; Marcus Görge Ullisch; Mingdong Dong; Niels Christian Nielsen; Jørgen Kjems

doi:10.1016/j.carbpol.2015.09.076

Chitosan-coated Poly(lactic-co-glycolic acid) Perfluorooctyl Bromide Nanoparticles for Cell Labeling in 19F Magnetic Resonance Imaging

Hieu Vu Quang, Mads Sloth Vinding, Dan Xia, Thomas Nielsen, Marcus Görge Ullisch, Mingdong Dong, Niels Christian Nielsen, Jørgen Kjems

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

29 Citations (Scopus)

Abstract

Noninvasive therapeutic cell tracking methods in living animals are important for understanding cell function and fate in connection with cell therapy. Here we report a new particle system based on chitosan-coated poly(lactic-co-glycolic acid) perfluorooctyl bromide (PLGA PFOB) nanoparticles designed for ¹⁹F magnetic resonance imaging (MRI) cell tracking. Chitosan was adsorbed onto the PLGA PFOB nanoparticles through electric interactions, which led to an increase in the hydrodynamic size and a surface charge proportional to the coating weight ratio. Confocal laser scanning microscopy, flow cytometry analysis and ¹⁹F-MRI showed that to achieve the highest labeling efficiency in vitro, the optimal weight ratio of chitosan to the PLGA PFOB nanoparticles was 1:10 for human mesenchymal stem cells (hMSCs) and 1:100 for Raw 264.7 macrophages. In vivo ¹⁹F-MRI showed that ¹⁹F labeled hMSCs remained at the injected site 24 h after injection. Thus, this study validates that chitosan-coated PLGA PFOB nanoparticles have the potential to track cell migration in vivo.

Original language	English
Journal	Carbohydrate Polymers
Volume	136
Pages (from-to)	936-944
Number of pages	9
ISSN	0144-8617
DOIs	https://doi.org/10.1016/j.carbpol.2015.09.076
Publication status	Published - 20 Jan 2016

Keywords

Chitosan
F-MRI
PFOB
PLGA

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10.1016/j.carbpol.2015.09.076

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@article{475d973b066a4d268d43a49475efb642,

title = "Chitosan-coated Poly(lactic-co-glycolic acid) Perfluorooctyl Bromide Nanoparticles for Cell Labeling in 19F Magnetic Resonance Imaging",

abstract = "Noninvasive therapeutic cell tracking methods in living animals are important for understanding cell function and fate in connection with cell therapy. Here we report a new particle system based on chitosan-coated poly(lactic-co-glycolic acid) perfluorooctyl bromide (PLGA PFOB) nanoparticles designed for 19F magnetic resonance imaging (MRI) cell tracking. Chitosan was adsorbed onto the PLGA PFOB nanoparticles through electric interactions, which led to an increase in the hydrodynamic size and a surface charge proportional to the coating weight ratio. Confocal laser scanning microscopy, flow cytometry analysis and 19F-MRI showed that to achieve the highest labeling efficiency in vitro, the optimal weight ratio of chitosan to the PLGA PFOB nanoparticles was 1:10 for human mesenchymal stem cells (hMSCs) and 1:100 for Raw 264.7 macrophages. In vivo 19F-MRI showed that 19F labeled hMSCs remained at the injected site 24 h after injection. Thus, this study validates that chitosan-coated PLGA PFOB nanoparticles have the potential to track cell migration in vivo.",

keywords = "Chitosan, F-MRI, PFOB, PLGA",

author = "{Vu Quang}, Hieu and Vinding, {Mads Sloth} and Dan Xia and Thomas Nielsen and Ullisch, {Marcus G{\"o}rge} and Mingdong Dong and Nielsen, {Niels Christian} and J{\o}rgen Kjems",

year = "2016",

month = jan,

day = "20",

doi = "10.1016/j.carbpol.2015.09.076",

language = "English",

volume = "136",

pages = "936--944",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Elsevier Ltd",

}

Chitosan-coated Poly(lactic-co-glycolic acid) Perfluorooctyl Bromide Nanoparticles for Cell Labeling in 19F Magnetic Resonance Imaging. / Vu Quang, Hieu; Vinding, Mads Sloth; Xia, Dan et al.
In: Carbohydrate Polymers, Vol. 136, 20.01.2016, p. 936-944.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Chitosan-coated Poly(lactic-co-glycolic acid) Perfluorooctyl Bromide Nanoparticles for Cell Labeling in 19F Magnetic Resonance Imaging

AU - Vu Quang, Hieu

AU - Vinding, Mads Sloth

AU - Xia, Dan

AU - Nielsen, Thomas

AU - Ullisch, Marcus Görge

AU - Dong, Mingdong

AU - Nielsen, Niels Christian

AU - Kjems, Jørgen

PY - 2016/1/20

Y1 - 2016/1/20

N2 - Noninvasive therapeutic cell tracking methods in living animals are important for understanding cell function and fate in connection with cell therapy. Here we report a new particle system based on chitosan-coated poly(lactic-co-glycolic acid) perfluorooctyl bromide (PLGA PFOB) nanoparticles designed for 19F magnetic resonance imaging (MRI) cell tracking. Chitosan was adsorbed onto the PLGA PFOB nanoparticles through electric interactions, which led to an increase in the hydrodynamic size and a surface charge proportional to the coating weight ratio. Confocal laser scanning microscopy, flow cytometry analysis and 19F-MRI showed that to achieve the highest labeling efficiency in vitro, the optimal weight ratio of chitosan to the PLGA PFOB nanoparticles was 1:10 for human mesenchymal stem cells (hMSCs) and 1:100 for Raw 264.7 macrophages. In vivo 19F-MRI showed that 19F labeled hMSCs remained at the injected site 24 h after injection. Thus, this study validates that chitosan-coated PLGA PFOB nanoparticles have the potential to track cell migration in vivo.

AB - Noninvasive therapeutic cell tracking methods in living animals are important for understanding cell function and fate in connection with cell therapy. Here we report a new particle system based on chitosan-coated poly(lactic-co-glycolic acid) perfluorooctyl bromide (PLGA PFOB) nanoparticles designed for 19F magnetic resonance imaging (MRI) cell tracking. Chitosan was adsorbed onto the PLGA PFOB nanoparticles through electric interactions, which led to an increase in the hydrodynamic size and a surface charge proportional to the coating weight ratio. Confocal laser scanning microscopy, flow cytometry analysis and 19F-MRI showed that to achieve the highest labeling efficiency in vitro, the optimal weight ratio of chitosan to the PLGA PFOB nanoparticles was 1:10 for human mesenchymal stem cells (hMSCs) and 1:100 for Raw 264.7 macrophages. In vivo 19F-MRI showed that 19F labeled hMSCs remained at the injected site 24 h after injection. Thus, this study validates that chitosan-coated PLGA PFOB nanoparticles have the potential to track cell migration in vivo.

KW - Chitosan

KW - F-MRI

KW - PFOB

KW - PLGA

U2 - 10.1016/j.carbpol.2015.09.076

DO - 10.1016/j.carbpol.2015.09.076

M3 - Journal article

C2 - 26572432

SN - 0144-8617

VL - 136

SP - 936

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JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Chitosan-coated Poly(lactic-co-glycolic acid) Perfluorooctyl Bromide Nanoparticles for Cell Labeling in 19F Magnetic Resonance Imaging

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Keywords

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