Chemical modification of small interfering RNA

Jesper Bertram Bramsen; Jørgen Kjems

doi:10.1007/978-1-61779-037-9_5

Chemical modification of small interfering RNA

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Abstract

Chemically synthesized siRNAs are widely used for gene silencing. For in vitro applications, stability, delivery, and immunological issues are rarely problematic, but for in vivo applications the situation is different. Limited stability, undesirable pharmacokinetic behaviour, and unanticipated side effects from the immune system call for more careful structural siRNA design and inclusion of chemical modifications at selected positions. Also the notion that siRNA induces significant off-target silencing of many non-related genes has promted new effective measures to enhance specificity. The scope of this review is to provide a simple guide to successful chemical and structural modification of siRNAs with improved activity, stability, specificity, and low toxicity.

Original language	English
Journal	Methods in Molecular Biology
Volume	721
Issue	1
Pages (from-to)	77-103
Number of pages	27
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-61779-037-9_5
Publication status	Published - 2011

Keywords

Animals
Drug Design
Humans
RNA, Small Interfering
Ribonucleases

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10.1007/978-1-61779-037-9_5

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KW - Animals

KW - Drug Design

KW - Humans

KW - RNA, Small Interfering

KW - Ribonucleases

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Chemical modification of small interfering RNA

Abstract

Keywords

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