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Characterization of the bioactivities of an ethanol extract and some of its constituents from the New Zealand native mushroom Hericium novae-zealandiae

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DOI

  • Zhixia Chen, Food Science, School of Chemical Sciences, The University of Auckland, Auckland 1010, New Zealand
  • ,
  • Karen Suzanne Bishop, Auckland Cancer Society Research Centre, Faculty of Medical, Discipline of Nutrition and Dietetics, School of Medical Sciences, Faculty of Medical and Health Sciences, The University of Auckland, Auckland 1010, New Zealand
  • ,
  • Hartono Tanambell
  • Peter Buchanan, Manaaki Whenua-Landcare Research, Auckland 1072, New Zealand
  • ,
  • Chris Smith, Manaaki Whenua-Landcare Research, Auckland 1072, New Zealand
  • ,
  • Siew Young Quek, Food Science, School of Chemical Sciences, The University of Auckland, Auckland 1010, New Zealand, Riddet Institute, New Zealand Centre of Research Excellence for Food Research, Palmerston North 4474, New Zealand
In this study, we investigated the potential bioactivities of an ethanol extract of Hericium novae-zealandiae and four of its constituents, namely hericenone C, hericene B, ergosterol and ergosterol peroxide. The proliferation of three prostate cancer cell lines, namely DU145, LNCaP and PC3, was evaluated after treatment with the extract and constituents. It was found that both the ethanol extract and ergosterol peroxide possess anti-proliferative activities to the three prostate cancer cell lines. Ergosterol peroxide was considered likely to be one of the major compounds responsible for the anti-proliferative effect of the ethanol extract. Subsequently, the results of RT-qPCR assay showed two possible mechanisms for these anti-proliferative activities. One is apoptosis, supported by the up-regulation of CASP3, CASP8, CASP9, and an increase in the ratio of Bax/Bcl2. The other is anti-inflammation, indicated by the down-regulation of IL6 and up-regulation of IL24. The ethanol extract also exhibited antioxidant and AChE inhibitory (though weak) activities. However, none of the four compounds were found to account for these latter two activities. This is the first report of the bioactivities, and the corresponding active ingredients of lipophilic constituents from H. novae-zealandiae.
Original languageEnglish
JournalFood & Function
Volume10
Issue10
Pages (from-to)6633-6643
Number of pages11
ISSN2042-6496
DOIs
Publication statusPublished - Oct 2019
Externally publishedYes

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