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Characterization of circular RNA concatemers

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Circular RNAs (circRNAs) constitute a novel subset in the fascinating world of noncoding RNA, and they are found in practically all eukaryotes. Most of them exhibit low expression levels but some are extremely abundant. Typically, circRNAs are studied by RT-PCR-based assays, but for certain types of analyses this technique is not suitable. Circular RNA with repetitive exons (circular concatemers) has been observed by us and others when transiently expressing circRNAs in cells, however techniques and assays to study these species have not been established. Here, this chapter outlines three biochemical assays (RNase R-, RNase H-, and alkaline-treatment) that combined with northern blotting are useful to study circRNAs in general and circular RNA concatemers in particular.

Original languageEnglish
Title of host publicationCircular RNAs : Methods and Protocols
EditorsC. Dietrerich, A. Papantonis
Number of pages15
Place of publicationNew York
PublisherHumana Press
Publication year11 Jan 2018
ISBN (print)978-1-4939-7561-7
ISBN (Electronic)978-1-4939-7562-4
Publication statusPublished - 11 Jan 2018
SeriesMethods in Molecular Biology

    Research areas

  • Alkaline treatment, Circular RNA, Northern blotting, RNA concatemers, RNAse H, RNAse R

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