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Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses.

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  • Department of Medical Microbiology and Immunology
  • Department of Molecular Biology
Rat monoclonal antibodies (MoAbs) against mouse mannan-binding lectin (MBL)-A and MBL-C were generated and assays for MBL-A and MBL-C were constructed. This allowed for the quantitative analysis of both proteins for the first time. Previously only MBL-A has been quantified using less standardized methods. In a mouse serum pool the concentrations were now determined at 7.5 microg MBL-A and 45 microg MBL-C per ml. On gel permeation chromatography of mouse serum, MBL-A eluted corresponding to a M(r) of 850 kDa whereas the majority of MBL-C eluted corresponding to a Mr of 950 kDa. On sucrose density gradient centrifugation the sedimentation velocities of MBL-A and MBL-C were estimated at 7.3 S and 10.8 S, respectively. The MBL-A and MBL-C levels in 10 laboratory mice strains were compared and found to vary between 4 microg/ml to 12 microg/ml, and 16 microg/ml to 118 microg/ml, respectively. After the induction of acute phase responses by intraperitoneal injection of either casein or lipopolysaccharide (LPS), MBL-A was found to increase approximately two-fold, with a maximum after 32 h, while MBL-C did not increase significantly. In comparison, serum amyloid A component (SAA) peaked at 15 h with an approximate 100-fold increase.
Udgivelsesdato: 2001-May
Original languageEnglish
JournalScandinavian Journal of Immunology
Pages (from-to)489-97
Number of pages8
Publication statusPublished - 2001

    Research areas

  • Acute-Phase Proteins, Acute-Phase Reaction, Animals, Blotting, Western, Carrier Proteins, Centrifugation, Density Gradient, Chromatography, Gel, Collectins, Female, Lectins, Male, Mice, Mice, Inbred Strains, Rats, Rats, Wistar

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