Changes in spatiotemporal protein and amino acid gradients in wheat caryopsis after N-topdressing

Yingxin Zhong; Nanna Hjort Vidkjær; Julio A. Massange-Sanchez; Bente Birgitte Laursen; René Gislum; Søren Borg; Dong Jiang; Kim Henrik Hebelstrup

doi:10.1016/j.plantsci.2019.110336

Changes in spatiotemporal protein and amino acid gradients in wheat caryopsis after N-topdressing

Yingxin Zhong, Nanna Hjort Vidkjær, Julio A. Massange-Sanchez, Bente Birgitte Laursen, René Gislum, Søren Borg, Dong Jiang^*, Kim Henrik Hebelstrup

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

13 Citations (Scopus)

Abstract

Wheat grain nitrogen content displays large variations within different pearling fractions of grains because of radial gradients in the protein content. We identified how spatiotemporal mechanisms regulate this. The protein gradients emerged clearly at 19 days after anthesis, with the highest N content in aleurone and seed coat, followed by outer endosperm, whereas the lowest was in middle and inner endosperm. Laser microdissection, qRT-PCR and LC–MS were used to dissect tissue from aleurone, outer endosperm, middle endosperm, inner endosperm and transfer cells, measure gene expression and levels of free and protein-bound amino acids, respectively. The results showed that different FAA transportation pathways worked in parallel during grain filling stage while the grain protein gradient did not follow spatial expression of storage proteins. Additionally, two nitrogen (N) topdressing timings were conducted, either at the emergence of top third leaf (standard timing) or top first leaf (delayed timing), finding that delayed N topdressing enhanced both amino acids supply and protein synthesis capacity. The results provide insight into protein synthesis and amino acid transport pathways in endosperm and suggest targets for the enhancement of specialty pearled wheat with higher quality.

Original language	English
Article number	110336
Journal	Plant Science
Volume	291
Number of pages	10
ISSN	0168-9452
DOIs	https://doi.org/10.1016/j.plantsci.2019.110336
Publication status	Published - 2020

Keywords

Amino acids
Gene expression profiling
Laser capture microdissection
Spatial distribution
Wheat caryopsis

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10.1016/j.plantsci.2019.110336

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@article{72280167382647a5942e5f800190683e,

title = "Changes in spatiotemporal protein and amino acid gradients in wheat caryopsis after N-topdressing",

abstract = "Wheat grain nitrogen content displays large variations within different pearling fractions of grains because of radial gradients in the protein content. We identified how spatiotemporal mechanisms regulate this. The protein gradients emerged clearly at 19 days after anthesis, with the highest N content in aleurone and seed coat, followed by outer endosperm, whereas the lowest was in middle and inner endosperm. Laser microdissection, qRT-PCR and LC–MS were used to dissect tissue from aleurone, outer endosperm, middle endosperm, inner endosperm and transfer cells, measure gene expression and levels of free and protein-bound amino acids, respectively. The results showed that different FAA transportation pathways worked in parallel during grain filling stage while the grain protein gradient did not follow spatial expression of storage proteins. Additionally, two nitrogen (N) topdressing timings were conducted, either at the emergence of top third leaf (standard timing) or top first leaf (delayed timing), finding that delayed N topdressing enhanced both amino acids supply and protein synthesis capacity. The results provide insight into protein synthesis and amino acid transport pathways in endosperm and suggest targets for the enhancement of specialty pearled wheat with higher quality.",

keywords = "Amino acids, Gene expression profiling, Laser capture microdissection, Spatial distribution, Wheat caryopsis",

author = "Yingxin Zhong and Vidkj{\ae}r, {Nanna Hjort} and Massange-Sanchez, {Julio A.} and Laursen, {Bente Birgitte} and Ren{\'e} Gislum and S{\o}ren Borg and Dong Jiang and Hebelstrup, {Kim Henrik}",

year = "2020",

doi = "10.1016/j.plantsci.2019.110336",

language = "English",

volume = "291",

journal = "Plant Science",

issn = "0168-9452",

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T1 - Changes in spatiotemporal protein and amino acid gradients in wheat caryopsis after N-topdressing

AU - Zhong, Yingxin

AU - Vidkjær, Nanna Hjort

AU - Massange-Sanchez, Julio A.

AU - Laursen, Bente Birgitte

AU - Gislum, René

AU - Borg, Søren

AU - Jiang, Dong

AU - Hebelstrup, Kim Henrik

PY - 2020

Y1 - 2020

N2 - Wheat grain nitrogen content displays large variations within different pearling fractions of grains because of radial gradients in the protein content. We identified how spatiotemporal mechanisms regulate this. The protein gradients emerged clearly at 19 days after anthesis, with the highest N content in aleurone and seed coat, followed by outer endosperm, whereas the lowest was in middle and inner endosperm. Laser microdissection, qRT-PCR and LC–MS were used to dissect tissue from aleurone, outer endosperm, middle endosperm, inner endosperm and transfer cells, measure gene expression and levels of free and protein-bound amino acids, respectively. The results showed that different FAA transportation pathways worked in parallel during grain filling stage while the grain protein gradient did not follow spatial expression of storage proteins. Additionally, two nitrogen (N) topdressing timings were conducted, either at the emergence of top third leaf (standard timing) or top first leaf (delayed timing), finding that delayed N topdressing enhanced both amino acids supply and protein synthesis capacity. The results provide insight into protein synthesis and amino acid transport pathways in endosperm and suggest targets for the enhancement of specialty pearled wheat with higher quality.

AB - Wheat grain nitrogen content displays large variations within different pearling fractions of grains because of radial gradients in the protein content. We identified how spatiotemporal mechanisms regulate this. The protein gradients emerged clearly at 19 days after anthesis, with the highest N content in aleurone and seed coat, followed by outer endosperm, whereas the lowest was in middle and inner endosperm. Laser microdissection, qRT-PCR and LC–MS were used to dissect tissue from aleurone, outer endosperm, middle endosperm, inner endosperm and transfer cells, measure gene expression and levels of free and protein-bound amino acids, respectively. The results showed that different FAA transportation pathways worked in parallel during grain filling stage while the grain protein gradient did not follow spatial expression of storage proteins. Additionally, two nitrogen (N) topdressing timings were conducted, either at the emergence of top third leaf (standard timing) or top first leaf (delayed timing), finding that delayed N topdressing enhanced both amino acids supply and protein synthesis capacity. The results provide insight into protein synthesis and amino acid transport pathways in endosperm and suggest targets for the enhancement of specialty pearled wheat with higher quality.

KW - Amino acids

KW - Gene expression profiling

KW - Laser capture microdissection

KW - Spatial distribution

KW - Wheat caryopsis

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DO - 10.1016/j.plantsci.2019.110336

M3 - Journal article

C2 - 31928684

AN - SCOPUS:85075569860

SN - 0168-9452

VL - 291

JO - Plant Science

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ER -

Changes in spatiotemporal protein and amino acid gradients in wheat caryopsis after N-topdressing

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Keywords

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