Changes in monocyte subsets are associated with clinical outcomes in severe malarial anaemia and cerebral malaria

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  • Jade Royo, PHARMADEV UMR 152, Institut de Recherche pour le Développement (IRD), Université Paul Sabatier Toulouse 3, Toulouse, France.
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  • Mouna Rahabi, PHARMADEV UMR 152, Institut de Recherche pour le Développement (IRD), Université Paul Sabatier Toulouse 3, Toulouse, France.
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  • Claire Kamaliddin, MERIT UMR 216, IRD, Université Paris 5, Sorbonne Paris Cité, Paris, France.
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  • Sem Ezinmegnon, Centre d'Etude et de Recherche sur le Paludisme Associé à la Grossesse et l'Enfance (CERPAGE), Cotonou, Benin. ezinmegnon.sem@gmail.com.
  • ,
  • David Olagnier
  • Hélène Authier, PHARMADEV UMR 152, Institut de Recherche pour le Développement (IRD), Université Paul Sabatier Toulouse 3, Toulouse, France.
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  • Achille Massougbodji, Centre d'Etude et de Recherche sur le Paludisme Associé à la Grossesse et l'Enfance (CERPAGE), Cotonou, Benin. ezinmegnon.sem@gmail.com.
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  • Jules Alao, Paediatric Department, Mother and Child University and Hospital Center (CHUMEL), Cotonou, Benin.
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  • Yélé Ladipo, Paediatric Department, Mother and Child University and Hospital Center (CHUMEL), Cotonou, Benin.
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  • Philippe Deloron, MERIT UMR 216, IRD, Université Paris 5, Sorbonne Paris Cité, Paris, France.
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  • Gwladys Bertin, MERIT UMR 216, IRD, Université Paris 5, Sorbonne Paris Cité, Paris, France.
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  • Bernard Pipy, PHARMADEV UMR 152, Institut de Recherche pour le Développement (IRD), Université Paul Sabatier Toulouse 3, Toulouse, France.
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  • Agnès Coste, PHARMADEV UMR 152, Institut de Recherche pour le Développement (IRD), Université Paul Sabatier Toulouse 3, Toulouse, France.
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  • Agnès Aubouy, PHARMADEV UMR 152, Institut de Recherche pour le Développement (IRD), Université Paul Sabatier Toulouse 3, Toulouse, France. agnes.aubouy@ird.fr.

Monocytes are plastic heterogeneous immune cells involved in host-parasite interactions critical for malaria pathogenesis. Human monocytes have been subdivided into three populations based on surface expression of CD14 and CD16. We hypothesised that proportions and phenotypes of circulating monocyte subsets can be markers of severity or fatality in children with malaria. To address this question, we compared monocytes sampled in children with uncomplicated malaria, severe malarial anaemia, or cerebral malaria. Flow cytometry was used to distinguish and phenotype monocyte subsets through CD14, CD16, CD36 and TLR2 expression. Data were first analysed by univariate analysis to evaluate their link to severity and death. Second, multinomial logistic regression was used to measure the specific effect of monocyte proportions and phenotypes on severity and death, after adjustments for other variables unrelated to monocytes. Multivariate analysis demonstrated that decreased percentages of non-classical monocytes were associated with death, suggesting that this monocyte subset has a role in resolving malaria. Using univariate analysis, we also showed that the role of non-classical monocytes involves a mostly anti-inflammatory profile and the expression of CD16. Further studies are needed to decipher the functions of this sub-population during severe malaria episodes, and understand the underlying mechanisms.

Original languageEnglish
Article number17545
JournalScientific Reports
Volume9
Issue1
Number of pages13
ISSN2045-2322
DOIs
Publication statusPublished - 2019

    Research areas

  • FALCIPARUM-PARASITIZED ERYTHROCYTES, CD14(+) CD16(+) MONOCYTES, CELL DEFORMABILITY, AFRICAN CHILDREN, BLOOD MONOCYTES, CD36, SUBPOPULATION, PHAGOCYTOSIS, SUPPRESSION, EXPRESSION

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