Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering

Vivek Mann; Daniela Grimm; Thomas J Corydon; Marcus Krüger; Markus Wehland; Stefan Riwaldt; Jayashree Sahana; Sascha Kopp; Johann Bauer; Janne E Reseland; Manfred Infanger; Aina Mari Lian; Elvis Okoro; Alamelu Sundaresan

doi:10.3390/ijms20061357

Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering

Vivek Mann, Daniela Grimm, Thomas J Corydon, Marcus Krüger, Markus Wehland, Stefan Riwaldt, Jayashree Sahana, Sascha Kopp, Johann Bauer, Janne E Reseland, Manfred Infanger, Aina Mari Lian, Elvis Okoro, Alamelu Sundaresan

Department of Biomedicine - Forskning og uddannelse, Øst

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (RPM) for 7 days and 14 days, with the purpose of investigating the effects of s-µg on biological processes and to engineer 3D bone constructs. RPM exposure of the hFOB 1.19 cells induces alterations in the cytoskeleton, cell adhesion, extra cellular matrix (ECM) and the 3D multicellular spheroid (MCS) formation. In addition, after 7 days, it influences the morphological appearance of these cells, as it forces adherent cells to detach from the surface and assemble into 3D structures. The RPM-exposed hFOB 1.19 cells exhibited a differential gene expression of the following genes: transforming growth factor beta 1 (TGFB1, bone morphogenic protein 2 (BMP2), SRY-Box 9 (SOX9), actin beta (ACTB), beta tubulin (TUBB), vimentin (VIM), laminin subunit alpha 1 (LAMA1), collagen type 1 alpha 1 (COL1A1), phosphoprotein 1 (SPP1) and fibronectin 1 (FN1). RPM exposure also induced a significantly altered release of the cytokines and bone biomarkers sclerostin (SOST), osteocalcin (OC), osteoprotegerin (OPG), osteopontin (OPN), interleukin 1 beta (IL-1β) and tumour necrosis factor 1 alpha (TNF-1α). After the two-week RPM exposure, the spheroids presented a bone-specific morphology. In conclusion, culturing cells in s-µg under gravitational unloading represents a novel technology for tissue-engineering of bone constructs and it can be used for investigating the mechanisms behind spaceflight-related bone loss as well as bone diseases such as osteonecrosis or bone injuries.

Original language	English
Article number	1357
Journal	International Journal of Molecular Sciences
Volume	20
Issue	6
ISSN	1661-6596
DOIs	https://doi.org/10.3390/ijms20061357
Publication status	Published - 2019

Keywords

Biomarker
Bone
Cytoskeleton
Osteoblasts
Simulated microgravity
Tissue engineering

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10.3390/ijms20061357Licence: CC BY

Cite this

Mann, V., Grimm, D., Corydon, T. J., Krüger, M., Wehland, M., Riwaldt, S., Sahana, J., Kopp, S., Bauer, J., Reseland, J. E., Infanger, M., Mari Lian, A., Okoro, E., & Sundaresan, A. (2019). Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering. International Journal of Molecular Sciences , 20(6), Article 1357. https://doi.org/10.3390/ijms20061357

@article{698fbf5d553145cda3b08010b3df299c,

title = "Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering",

abstract = "Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (RPM) for 7 days and 14 days, with the purpose of investigating the effects of s-µg on biological processes and to engineer 3D bone constructs. RPM exposure of the hFOB 1.19 cells induces alterations in the cytoskeleton, cell adhesion, extra cellular matrix (ECM) and the 3D multicellular spheroid (MCS) formation. In addition, after 7 days, it influences the morphological appearance of these cells, as it forces adherent cells to detach from the surface and assemble into 3D structures. The RPM-exposed hFOB 1.19 cells exhibited a differential gene expression of the following genes: transforming growth factor beta 1 (TGFB1, bone morphogenic protein 2 (BMP2), SRY-Box 9 (SOX9), actin beta (ACTB), beta tubulin (TUBB), vimentin (VIM), laminin subunit alpha 1 (LAMA1), collagen type 1 alpha 1 (COL1A1), phosphoprotein 1 (SPP1) and fibronectin 1 (FN1). RPM exposure also induced a significantly altered release of the cytokines and bone biomarkers sclerostin (SOST), osteocalcin (OC), osteoprotegerin (OPG), osteopontin (OPN), interleukin 1 beta (IL-1β) and tumour necrosis factor 1 alpha (TNF-1α). After the two-week RPM exposure, the spheroids presented a bone-specific morphology. In conclusion, culturing cells in s-µg under gravitational unloading represents a novel technology for tissue-engineering of bone constructs and it can be used for investigating the mechanisms behind spaceflight-related bone loss as well as bone diseases such as osteonecrosis or bone injuries.",

keywords = "Biomarker, Bone, Cytoskeleton, Osteoblasts, Simulated microgravity, Tissue engineering",

author = "Vivek Mann and Daniela Grimm and Corydon, {Thomas J} and Marcus Kr{\"u}ger and Markus Wehland and Stefan Riwaldt and Jayashree Sahana and Sascha Kopp and Johann Bauer and Reseland, {Janne E} and Manfred Infanger and {Mari Lian}, Aina and Elvis Okoro and Alamelu Sundaresan",

year = "2019",

doi = "10.3390/ijms20061357",

language = "English",

volume = "20",

journal = "International Journal of Molecular Sciences ",

issn = "1661-6596",

publisher = "MDPI AG",

number = "6",

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Mann, V, Grimm, D , Corydon, TJ, Krüger, M, Wehland, M, Riwaldt, S, Sahana, J, Kopp, S, Bauer, J, Reseland, JE, Infanger, M, Mari Lian, A, Okoro, E & Sundaresan, A 2019, 'Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering', International Journal of Molecular Sciences , vol. 20, no. 6, 1357. https://doi.org/10.3390/ijms20061357

Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering. / Mann, Vivek; Grimm, Daniela ; Corydon, Thomas J et al.
In: International Journal of Molecular Sciences , Vol. 20, No. 6, 1357, 2019.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering

AU - Mann, Vivek

AU - Grimm, Daniela

AU - Corydon, Thomas J

AU - Krüger, Marcus

AU - Wehland, Markus

AU - Riwaldt, Stefan

AU - Sahana, Jayashree

AU - Kopp, Sascha

AU - Bauer, Johann

AU - Reseland, Janne E

AU - Infanger, Manfred

AU - Mari Lian, Aina

AU - Okoro, Elvis

AU - Sundaresan, Alamelu

PY - 2019

Y1 - 2019

N2 - Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (RPM) for 7 days and 14 days, with the purpose of investigating the effects of s-µg on biological processes and to engineer 3D bone constructs. RPM exposure of the hFOB 1.19 cells induces alterations in the cytoskeleton, cell adhesion, extra cellular matrix (ECM) and the 3D multicellular spheroid (MCS) formation. In addition, after 7 days, it influences the morphological appearance of these cells, as it forces adherent cells to detach from the surface and assemble into 3D structures. The RPM-exposed hFOB 1.19 cells exhibited a differential gene expression of the following genes: transforming growth factor beta 1 (TGFB1, bone morphogenic protein 2 (BMP2), SRY-Box 9 (SOX9), actin beta (ACTB), beta tubulin (TUBB), vimentin (VIM), laminin subunit alpha 1 (LAMA1), collagen type 1 alpha 1 (COL1A1), phosphoprotein 1 (SPP1) and fibronectin 1 (FN1). RPM exposure also induced a significantly altered release of the cytokines and bone biomarkers sclerostin (SOST), osteocalcin (OC), osteoprotegerin (OPG), osteopontin (OPN), interleukin 1 beta (IL-1β) and tumour necrosis factor 1 alpha (TNF-1α). After the two-week RPM exposure, the spheroids presented a bone-specific morphology. In conclusion, culturing cells in s-µg under gravitational unloading represents a novel technology for tissue-engineering of bone constructs and it can be used for investigating the mechanisms behind spaceflight-related bone loss as well as bone diseases such as osteonecrosis or bone injuries.

AB - Human cells, when exposed to both real and simulated microgravity (s-µg), form 3D tissue constructs mirroring in vivo architectures (e.g., cartilage, intima constructs, cancer spheroids and others). In this study, we exposed human foetal osteoblast (hFOB 1.19) cells to a Random Positioning Machine (RPM) for 7 days and 14 days, with the purpose of investigating the effects of s-µg on biological processes and to engineer 3D bone constructs. RPM exposure of the hFOB 1.19 cells induces alterations in the cytoskeleton, cell adhesion, extra cellular matrix (ECM) and the 3D multicellular spheroid (MCS) formation. In addition, after 7 days, it influences the morphological appearance of these cells, as it forces adherent cells to detach from the surface and assemble into 3D structures. The RPM-exposed hFOB 1.19 cells exhibited a differential gene expression of the following genes: transforming growth factor beta 1 (TGFB1, bone morphogenic protein 2 (BMP2), SRY-Box 9 (SOX9), actin beta (ACTB), beta tubulin (TUBB), vimentin (VIM), laminin subunit alpha 1 (LAMA1), collagen type 1 alpha 1 (COL1A1), phosphoprotein 1 (SPP1) and fibronectin 1 (FN1). RPM exposure also induced a significantly altered release of the cytokines and bone biomarkers sclerostin (SOST), osteocalcin (OC), osteoprotegerin (OPG), osteopontin (OPN), interleukin 1 beta (IL-1β) and tumour necrosis factor 1 alpha (TNF-1α). After the two-week RPM exposure, the spheroids presented a bone-specific morphology. In conclusion, culturing cells in s-µg under gravitational unloading represents a novel technology for tissue-engineering of bone constructs and it can be used for investigating the mechanisms behind spaceflight-related bone loss as well as bone diseases such as osteonecrosis or bone injuries.

KW - Biomarker

KW - Bone

KW - Cytoskeleton

KW - Osteoblasts

KW - Simulated microgravity

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85063251532&partnerID=8YFLogxK

U2 - 10.3390/ijms20061357

DO - 10.3390/ijms20061357

M3 - Journal article

C2 - 30889841

SN - 1661-6596

VL - 20

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 6

M1 - 1357

ER -

Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering

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Keywords

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