TY - JOUR
T1 - Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries
AU - Raniolo, Sofia
AU - Croce, Stefano
AU - Thomsen, Rasmus P.
AU - Okholm, Anders H.
AU - Unida, Valeria
AU - Iacovelli, Federico
AU - Manetto, Antonio
AU - Kjems, Jørgen
AU - Desideri, Alessandro
AU - Biocca, Silvia
PY - 2019/6
Y1 - 2019/6
N2 - DNA nanostructures with different sizes and shapes, assembled through either covalent or non-covalent bonds, namely tetrahedral and octahedral nanocages, rod-shaped chainmails, square box and rectangular DNA origami structures, were compared for their stability in serum, cell surface binding, internalization efficiency, and intracellular degradation rate. For cell internalization a specific cell system, highly expressing the scavenger receptor LOX-1 was used. The results indicate that LOX-1 binds and internalizes a broad family of DNA structures of different sizes that, however, have a different fate and lifetime inside the cells. Covalently linked tetrahedra, octahedra or chainmails are intact inside cells for up to 18 hours whilst the same DNA nanostructures without covalent bonds along with square box and rectangular origami are rapidly degraded. These data suggest that non-covalently linked structures may be useful for fast drug release whilst the covalently-linked structures could be appropriate vehicles for slow release of molecules.
AB - DNA nanostructures with different sizes and shapes, assembled through either covalent or non-covalent bonds, namely tetrahedral and octahedral nanocages, rod-shaped chainmails, square box and rectangular DNA origami structures, were compared for their stability in serum, cell surface binding, internalization efficiency, and intracellular degradation rate. For cell internalization a specific cell system, highly expressing the scavenger receptor LOX-1 was used. The results indicate that LOX-1 binds and internalizes a broad family of DNA structures of different sizes that, however, have a different fate and lifetime inside the cells. Covalently linked tetrahedra, octahedra or chainmails are intact inside cells for up to 18 hours whilst the same DNA nanostructures without covalent bonds along with square box and rectangular origami are rapidly degraded. These data suggest that non-covalently linked structures may be useful for fast drug release whilst the covalently-linked structures could be appropriate vehicles for slow release of molecules.
UR - https://www.scopus.com/pages/publications/85067057635
U2 - 10.1039/c9nr02006c
DO - 10.1039/c9nr02006c
M3 - Journal article
C2 - 31134260
AN - SCOPUS:85067057635
SN - 2040-3364
VL - 11
SP - 10808
EP - 10818
JO - Nanoscale
JF - Nanoscale
IS - 22
ER -