Cell Membrane Vesicle Camouflaged Artificial Cells

Paula De Dios Andres; Stefan Pendlmayr; Noga Gal; Cathrine Abild Meyer; Cecilie Ryberg; Olav Vestrheim; Noel Angelo Kalacas; Fatih Demir; Markus M. Rinschen; Karsten Haupt; Rajeshwar Prosad Mookerjee; Brigitte Städler

doi:10.1002/adfm.202424504

Cell Membrane Vesicle Camouflaged Artificial Cells

Paula De Dios Andres, Stefan Pendlmayr, Noga Gal, Cathrine Abild Meyer, Cecilie Ryberg, Olav Vestrheim, Noel Angelo Kalacas, Fatih Demir, Markus M. Rinschen, Karsten Haupt, Rajeshwar Prosad Mookerjee^*, Brigitte Städler^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

The integration of artificial and mammalian cells into semi-synthetic aggregates remains a challenge in bottom-up synthetic biology. Here, the fabrication of cell membrane vesicles (CMV) from HepG2 cells and their use as a coating for alginate microgels to produce camouflaged artificial cells (ACs) is demonstrated. These ACs are used for the assembly of either synthetic aggregates or semi-synthetic aggregates. In the first case, a predator-defendant and a liver-like synthetic aggregates are investigated, showing promising initial steps toward complex synthetic aggregates. In the other case, the camouflaged ACs show enhanced integration with HepG2 cells. The encapsulation of a reactive oxygen species (ROS) scavenger artificial enzyme in the ACs shows protection against tert-butyl hydroperoxide in terms of HepG2 cell viability, proliferation, and mitochondrial health in semi-synthetic aggregates. Taken together, this effort is a substantial step forward in combining mammalian cells and ACs in the same aggregate where the latter act as support units.

Original language	English
Journal	Advanced Functional Materials
ISSN	1616-301X
DOIs	https://doi.org/10.1002/adfm.202424504
Publication status	E-pub / Early view - 2025

Keywords

artificial cells
cell membrane vesicles
HepG2 cells
hydrogels
semi-synthetic aggregates

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10.1002/adfm.202424504

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@article{64448d2589c3450ebccda82c9eac0fd0,

title = "Cell Membrane Vesicle Camouflaged Artificial Cells",

abstract = "The integration of artificial and mammalian cells into semi-synthetic aggregates remains a challenge in bottom-up synthetic biology. Here, the fabrication of cell membrane vesicles (CMV) from HepG2 cells and their use as a coating for alginate microgels to produce camouflaged artificial cells (ACs) is demonstrated. These ACs are used for the assembly of either synthetic aggregates or semi-synthetic aggregates. In the first case, a predator-defendant and a liver-like synthetic aggregates are investigated, showing promising initial steps toward complex synthetic aggregates. In the other case, the camouflaged ACs show enhanced integration with HepG2 cells. The encapsulation of a reactive oxygen species (ROS) scavenger artificial enzyme in the ACs shows protection against tert-butyl hydroperoxide in terms of HepG2 cell viability, proliferation, and mitochondrial health in semi-synthetic aggregates. Taken together, this effort is a substantial step forward in combining mammalian cells and ACs in the same aggregate where the latter act as support units.",

keywords = "artificial cells, cell membrane vesicles, HepG2 cells, hydrogels, semi-synthetic aggregates",

author = "{De Dios Andres}, Paula and Stefan Pendlmayr and Noga Gal and {Abild Meyer}, Cathrine and Cecilie Ryberg and Olav Vestrheim and Kalacas, {Noel Angelo} and Fatih Demir and Rinschen, {Markus M.} and Karsten Haupt and Mookerjee, {Rajeshwar Prosad} and Brigitte St{\"a}dler",

note = "Publisher Copyright: {\textcopyright} 2025 The Author(s). Advanced Functional Materials published by Wiley-VCH GmbH.",

year = "2025",

doi = "10.1002/adfm.202424504",

language = "English",

journal = "Advanced Functional Materials",

issn = "1616-301X",

publisher = "Wiley-VCH Verlag",

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TY - JOUR

T1 - Cell Membrane Vesicle Camouflaged Artificial Cells

AU - De Dios Andres, Paula

AU - Pendlmayr, Stefan

AU - Gal, Noga

AU - Abild Meyer, Cathrine

AU - Ryberg, Cecilie

AU - Vestrheim, Olav

AU - Kalacas, Noel Angelo

AU - Demir, Fatih

AU - Rinschen, Markus M.

AU - Haupt, Karsten

AU - Mookerjee, Rajeshwar Prosad

AU - Städler, Brigitte

PY - 2025

Y1 - 2025

N2 - The integration of artificial and mammalian cells into semi-synthetic aggregates remains a challenge in bottom-up synthetic biology. Here, the fabrication of cell membrane vesicles (CMV) from HepG2 cells and their use as a coating for alginate microgels to produce camouflaged artificial cells (ACs) is demonstrated. These ACs are used for the assembly of either synthetic aggregates or semi-synthetic aggregates. In the first case, a predator-defendant and a liver-like synthetic aggregates are investigated, showing promising initial steps toward complex synthetic aggregates. In the other case, the camouflaged ACs show enhanced integration with HepG2 cells. The encapsulation of a reactive oxygen species (ROS) scavenger artificial enzyme in the ACs shows protection against tert-butyl hydroperoxide in terms of HepG2 cell viability, proliferation, and mitochondrial health in semi-synthetic aggregates. Taken together, this effort is a substantial step forward in combining mammalian cells and ACs in the same aggregate where the latter act as support units.

AB - The integration of artificial and mammalian cells into semi-synthetic aggregates remains a challenge in bottom-up synthetic biology. Here, the fabrication of cell membrane vesicles (CMV) from HepG2 cells and their use as a coating for alginate microgels to produce camouflaged artificial cells (ACs) is demonstrated. These ACs are used for the assembly of either synthetic aggregates or semi-synthetic aggregates. In the first case, a predator-defendant and a liver-like synthetic aggregates are investigated, showing promising initial steps toward complex synthetic aggregates. In the other case, the camouflaged ACs show enhanced integration with HepG2 cells. The encapsulation of a reactive oxygen species (ROS) scavenger artificial enzyme in the ACs shows protection against tert-butyl hydroperoxide in terms of HepG2 cell viability, proliferation, and mitochondrial health in semi-synthetic aggregates. Taken together, this effort is a substantial step forward in combining mammalian cells and ACs in the same aggregate where the latter act as support units.

KW - artificial cells

KW - cell membrane vesicles

KW - HepG2 cells

KW - hydrogels

KW - semi-synthetic aggregates

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U2 - 10.1002/adfm.202424504

DO - 10.1002/adfm.202424504

M3 - Journal article

AN - SCOPUS:85218696820

SN - 1616-301X

JO - Advanced Functional Materials

JF - Advanced Functional Materials

ER -

Cell Membrane Vesicle Camouflaged Artificial Cells

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Keywords

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